Comparison of modification sites in glycated crystallin in vitro and in vivo.

Glycation of α-crystallin is responsible for age- and diabetic-related cataracts, which are the main cause of blindness worldwide. We optimized the method of identification of lysine residues prone to glycation using the combination of LC-MS, isotopic labeling, and modified synthetic peptide standards with the glycated lysine derivative (Fmoc-Lys(i,i-Fru,Boc)-OH). The in vitro glycation of bovine lens α-crystallin was conducted by optimized method with the equimolar mixture of [(12)C6]- and [(13)C6]D-glucose.

PPARg2 Ala¹² variant protects against Graves' orbitopathy and modulates the course of the disease.

Orbital fibroblast differentiation to adipocytes is a peroxisome proliferator-activated receptor g (PPARg)-dependent process essential for pathogenic tissue remodeling in Graves' orbitopathy (GO). PPARg2 Pro¹²Ala polymorphism modulates expression and/or function of the molecule encoded by this gene and is a promising locus of GO. Here, we analyzed associations of PPARg2 Pro¹²Ala with clinical manifestation of GO in 742 Polish Caucasians including 276 Graves' disease (GD) patients.

[Intravitreal Bevacizumab injections in a child with choroidal neovascularization--case report].

A healthy 11 year old boy with active choroidal neovascularization (CNV) was referred to the Department of Ophthalmology in Wroclaw for unilateral visual deterioration from 2 weeks. The best-corrected visual acuity was counting fingers from 4 meters. Boy was successfully treated with three intravitreal injections of bevacizumab.