Genetic Heterogeneity of Strains Revealed by RFLP Analysis of Region and Gene.

PCR-RFLP targeting of the 16S rDNA and genes, as well as the region, was applied to identify and differentiate between the spoilage and non-spoilage species. Eight reference strains and 75 strains isolated from spoiled juices, juice concentrates, drinks, its intermediates, and fresh apples were subject to study. Hin6I restriction patterns of the 16S rDNA gene enabled distinguishing between all the species analyzed, while the gene and gene cluster analysis also revealed that there were two major types among the isolates, one similar to the reference strain DSM 2498, and the other similar to the reference strain ATCC 49025.

Germination and Inactivation of Alicyclobacillus acidoterrestris Spores Induced by Moderate Hydrostatic Pressure.

Given the importance of spoilage caused by Alicyclobacillus acidoterrestris for the fruit juice industry, the objective of this work was to study the germination and inactivation of A. acidoterrestris spores induced by moderate hydrostatic pressure. Hydrostatic pressure treatment can induce the germination and inactivation of A.