Role of Piezo1 in modulating the RANKL/OPG ratio in mouse osteoblast cells exposed to Porphyromonas gingivalis lipopolysaccharide and mechanical stress.

Aims: Excessive occlusal force with periodontitis leads to rapid alveolar bone resorption. However, the molecular mechanism by which inflammation and mechanical stress cause bone resorption remains unclear. We examined the role of Piezo1, a mechanosensitive ion channel expressed on osteoblasts, in the changes in the receptor activator of nuclear factor-kappa B ligand (RANKL)/osteoprotegerin (OPG) ratio in mouse MC3T3-E1 osteoblast-like cells under Porphyromonas gingivalis lipopolysaccharide (P.

Implant Site Development by Orthodontic Extrusion and Buccal Root Torque at a Site Showing Severe Gingival Recession with Periodontitis: A Case Report.

This case report describes alveolar ridge augmentation around a hopeless maxillary canine with severe buccal gingival recession and periodontitis using orthodontic extrusion and a 90-degree buccal root torque in preparation for implant placement. After the orthodontic therapy, the palatal surface of the canine root reached the top of the alveolar bone, parallel to the occlusal plane, with newly formed bone and keratinized mucosa. An implant was successfully placed without combined bone augmentation.

Effects of periodontal treatment on the medical status of patients with type 2 diabetes mellitus: a pilot study.

Background: Studies have demonstrated that periodontal disease is associated with the development of systemic complications in patients with type 2 diabetes mellitus (T2DM). The purpose of this pilot study was to investigate which markers among various systemic disease parameters are affected by periodontal treatment in patients with T2DM.

Methods: Twelve patients with T2DM were given oral hygiene instructions and subsequent subgingival scaling and root planing.

Lysine-specific proteolytic activity responsible for forsythia detaching factor modification.

Objectives: The objective of the present study was to clarify the lysine-specific proteolytic activity derived from periodontal pathogens responsible for Forsythia detaching factor (FDF) modification.

Design: The activity responsible for FDF modification in Tannerella forsythia and Porphyromonas gingivalis were evaluated by colorimetric assay using Ac-Arg-Ala-Lys-p-nitroaniline as a substrate. FDF modification in T.

Modification of forsythia detaching factor by gingival crevicular fluid in periodontitis.

Objectives: Forsythia detaching factor (FDF) is a virulence factor of Tannerella forsythia detected as a mixture of the 60-kDa form of FDF and the 28-kDa C-terminal fragment (FDFc). The objective of the present study was to clarify the proteolytic activity of gingival crevicular fluid (GCF) from patients with periodontitis and healthy subjects using recombinant FDF (rFDF) as substrate.

Design: Eleven patients with periodontitis and 6 healthy subjects were recruited.

The significance of podoplanin expression in human inflamed gingiva.

Podoplanin, a transmembrane glycoprotein, has been considered to be expressed specifically by lymphatic endothelial cells. However, recent studies have shown that the protein is expressed in a variety of normal as well as neoplastic tissues, and that its expression might be related to cell migration and invasion. In this study, we examined podoplanin expression in inflamed gingival tissues using an immunohistochemical method.

Hypoxia and reoxygenation augment bone-resorbing factor production from human periodontal ligament cells.

Background: Oxygen deficiency caused by occlusal trauma and smoking may be associated with bone resorption in periodontitis. In the present study, the effects of hypoxia and reoxygenation on the production of bone-resorbing factors by cultured human periodontal ligament (PDL) cells were examined.

Methods: Human PDL cells were cultured in 1% O(2) (hypoxia), 20% O(2) (normal oxygen tension [normoxia]), or an oxygen concentration that went from 1% to 20% (reoxygenation).

High prevalence of Helicobacter pylori detected by PCR in the oral cavities of periodontitis patients.

Background: Helicobacter pylori has been associated with the development of peptic ulcers and gastric cancer. Although it may be transmitted through the oral cavity, it is unknown whether the oral cavity acts as a permanent reservoir for this bacterium. The purpose of this study was to use nested polymerase chain reaction (PCR) to clarify whether the oral cavity acts as a reservoir for H.

Elevated kallikrein activity in plasma from stable liver transplant recipients.

Extensive in vitro conversion of complement components C3 and C4 has been observed in EDTA plasma obtained from a number of stable orthotopic liver transplant recipients (LTR) [Clin. Chem. 45 (1999) 1190].