An insect pheromone primes tolerance of herbivory in goldenrod plants.

Environmental cues that predict increased risk of herbivory can prime plant defenses; however, few studies have explored how such cues elicit broader plant responses, including potential effects on plant growth and other resource allocations that may affect tolerance to herbivore damage. We exposed goldenrod plants (Solidago altissima) to varying concentrations of the putative sex pheromone of a gall-inducing herbivore, which has previously been implicated in defense priming. In experiments with two plant genotypes and three herbivore populations, any level of exposure to the pheromone enhanced tolerance of galling, rescuing flower production to levels observed for ungalled plants.

Illuminating patterns of firefly abundance using citizen science data and machine learning models.

As insect populations decline in many regions, conservation biologists are increasingly tasked with identifying factors that threaten insect species and developing effective strategies for their conservation. One insect group of global conservation concern are fireflies (Coleoptera: Lampyridae). Although quantitative data on firefly populations are lacking for most species, anecdotal reports suggest that some firefly populations have declined in recent decades.

Abscisic Acid: A Potential Secreted Effector Synthesized by Phytophagous Insects for Host-Plant Manipulation.

Abscisic acid (ABA) is an isoprenoid-derived plant signaling molecule involved in a wide variety of plant processes, including facets of growth and development as well as responses to abiotic and biotic stress. ABA had previously been reported in a wide variety of animals, including insects and humans. We used high-performance liquid chromatography-electrospray ionization tandem mass spectrometry (HPLC-(ESI)-MS/MS) to examine concentrations of ABA in 17 species of phytophagous insects, including gall- and non-gall-inducing species from all insect orders with species known to induce plant galls: Thysanoptera, Hemiptera, Lepidoptera, Coleoptera, Diptera, and Hymenoptera.

Extreme acidity in a cynipid gall: a potential new defensive strategy against natural enemies.

The morphology of insect-induced galls contributes to defences of the gall-inducing insect species against its natural enemies. In terms of gall chemistry, the only defensive compounds thus far identified in galls are tannins that accumulate in many galls, preventing damage by herbivores. Intrigued by the fruit-like appearance of the translucent oak gall (TOG; , Cynipidae, Hymenoptera) induced on red oak (), we hypothesized that its chemical composition may deviate from other galls.

Adverse effects of inbreeding on the transgenerational expression of herbivore-induced defense traits in Solanum carolinense.

In addition to directly inducing physical and chemical defenses, herbivory experienced by plants in one generation can influence the expression of defensive traits in offspring. Plant defense phenotypes can be compromised by inbreeding, and there is some evidence that such adverse effects can extend to the transgenerational expression of induced resistance. We explored how the inbreeding status of maternal Solanum carolinense plants influenced the transgenerational effects of herbivory on the defensive traits and herbivore resistance of offspring.

More phylogenetically diverse polycultures inconsistently suppress insect herbivore populations.

Because the diet of many herbivorous insects is restricted to closely related taxa with similar chemistry, intercropping with diverse plant communities may reduce both pest populations and reliance on chemical pesticides in agroecosystems. We tested whether the effectiveness of intercropping against herbivorous insects depends on the phylogenetic relatedness of neighboring crops, using butternut squash (Cucurbita moschata) as a focal crop species in a series of different intercropping combinations. We found that increased phylogenetic divergence of neighboring plants could reduce abundance of herbivorous insects, but the effect was only detectable mid-season.

Early-season plant cover supports more effective pest control than insecticide applications.

Growing evidence suggests that conservation agricultural practices, like no-till and cover crops, help protect annual crops from insect pests by supporting populations of resident arthropod predators. While adoption of conservation practices is growing, most field crop producers are also using more insecticides, including neonicotinoid seed coatings, as insurance against early-season insect pests. This tactic may disrupt benefits associated with conservation practices by reducing arthropods that contribute to biological control.

Toxicity of clothianidin to common Eastern North American fireflies.

Background: Previous research suggests that fireflies (Coleoptera: Lampyridae) are susceptible to commonly used insecticides. In the United States, there has been a rapid and widespread adoption of neonicotinoid insecticides, predominantly used as seed coatings on large-acreage crops like corn, soy, and cotton. Neonicotinoid insecticides are persistent in soil yet mobile in water, so they have potential to contaminate firefly habitats both in and adjacent to application sites.

Insecticide-contaminated honeydew: risks for beneficial insects.

Honeydew is the sugar-rich excretion of phloem-feeding hemipteran insects such as aphids, mealybugs, whiteflies, and psyllids, and can be a main carbohydrate source for beneficial insects in some ecosystems. Recent research has revealed that water-soluble, systemic insecticides contaminate honeydew excreted by hemipterans that feed on plants treated with these insecticides. This contaminated honeydew can be toxic to beneficial insects, such as pollinators, parasitic wasps and generalist predators that feed on it.

Life History, Biology, and Distribution of Pterostichus melanarius (Coleoptera: Carabidae) in North America.

Pterostichus melanarius (Illiger, 1798) is a Palearctic generalist predator native to Europe. It was unintentionally introduced to North America at least twice in the mid 1920s and has since become widespread in Canada and the United States. Although P.

Small-Grain Cover Crops Have Limited Effect on Neonicotinoid Contamination from Seed Coatings.

Neonicotinoids from insecticidal seed coatings can contaminate soil in treated fields and adjacent areas, posing a potential risk to nontarget organisms and ecological function. To determine if cover crops can mitigate neonicotinoid contamination in treated and adjacent areas, we measured neonicotinoid concentrations for three years in no-till corn-soybean rotations, planted with or without neonicotinoid seed coatings, and with or without small grain cover crops. Although neonicotinoids were detected in cover crops, high early season dissipation provided little opportunity for winter-planted cover crops to absorb significant neonicotinoid residues; small grain cover crops failed to mitigated neonicotinoid contamination in either treated or untreated plots.

Newer characters, same story: neonicotinoid insecticides disrupt food webs through direct and indirect effects.

During the Green Revolution, older classes of insecticides contributed to biodiversity loss by decreasing insect populations and bioaccumulating across food webs. Introduction of Integrated Pest Management (IPM) improved stewardship of insecticides and promised fewer non-target effects. IPM adoption has waned in recent decades, and popularity of newer classes of insecticides, like the neonicotinoids, has surged, posing new and unique threats to insect populations.

Giant polyploid epidermal cells and male pheromone production in the tephritid fruit fly Eurosta solidaginis (Diptera: Tephritidae).

Eurosta solidaginis males produce large amounts of putative sex pheromone compared to other insect species; however, neither the site of pheromone production nor the release mechanism has been characterized. We compared E. solidaginis males and females, focusing on sexually dimorphic structures that are known to be involved in pheromone production in other tephritid species.

Wild bees as winners and losers: Relative impacts of landscape composition, quality, and climate.

Wild bees, like many other taxa, are threatened by land-use and climate change, which, in turn, jeopardizes pollination of crops and wild plants. Understanding how land-use and climate factors interact is critical to predicting and managing pollinator populations and ensuring adequate pollination services, but most studies have evaluated either land-use or climate effects, not both. Furthermore, bee species are incredibly variable, spanning an array of behavioral, physiological, and life-history traits that can increase or decrease resilience to land-use or climate change.

Assessing surface and subsurface transport of neonicotinoid insecticides from no-till crop fields.

Increased use of neonicotinoid-coated crop seeds introduces greater amounts of insecticides into the environment, where they are vulnerable to transport. To understand the transport of neonicotinoids from agricultural fields, we planted maize (Zea mays L.) seeds coated with thiamethoxam in lysimeter plots in central Pennsylvania.

The Evolution of Endophagy in Herbivorous Insects.

Herbivorous feeding inside plant tissues, or endophagy, is a common lifestyle across Insecta, and occurs in insect taxa that bore, roll, tie, mine, gall, or otherwise modify plant tissues so that the tissues surround the insects while they are feeding. Some researchers have developed hypotheses to explain the adaptive significance of certain endophytic lifestyles (e.g.

Ground Predator Activity-Density and Predation Rates Are Weakly Supported by Dry-Stack Cow Manure and Wheat Cover Crops in No-Till Maize.

Because it keeps land in production, conservation programs that focus on in-field habitat manipulations may help farmers better support predators than by building predator habitat around fields. We investigated two in-field habitat manipulations that benefit producers and soil quality: fertilizing with dry-stack cow manure and planting a wheat cover crop. We hypothesized that, compared with inorganic fertilizer and fallow plots, both treatments augment habitat and residue and support more small arthropods that can serve as alternative prey for larger predators.

Policies Restricting Overlapping Surgeries Negatively Impact Access to Care, Clinical Efficiency, and Hospital Revenue: A Forecasting Model for Surgical Scheduling.

Objective: To model the financial impact of policies governing the scheduling of overlapping surgeries, and to identify optimal solutions that maximize operating efficiency that satisfy the fiduciary duty to patients.

Background: Hospitals depend on procedural revenue to maintain financial health as the recent pandemic has revealed. Proposed policies governing the scheduling of overlapping surgeries may dramatically impact hospital revenue.

Parasitoids, Nematodes, and Protists in Populations of Striped Cucumber Beetle (Coleoptera: Chrysomelidae).

The striped cucumber beetle, Acalymma vittatum (Fabricius), is an important pest of cucurbit production in the eastern United States, where most commercial producers rely on insecticides to control this pest species. Biological control provides an alternative to insecticide use, but for A. vittatum, top-down control has not been well developed.

The Influence of Marking Methods on Mobility, Survivorship, and Field Recovery of Halyomorpha halys (Hemiptera: Pentatomidae) Adults and Nymphs.

Halyomorpha halys (Stål), the brown marmorated stink bug, is an invasive and highly polyphagous insect that has caused serious economic injury to specialty and row crops in the United States and Europe. Here, we evaluated the effects of marking adult and nymphal H. halys with four different colors of fluorescent powder (Blaze Orange, Corona Pink, Horizon Blue, and Signal Green) on mobility and survivorship in laboratory bioassays.

A long-term dataset on wild bee abundance in Mid-Atlantic United States.

With documented global declines in insects, including wild bees, there has been increasing interest in developing and expanding insect monitoring programs. Our objective here was to organize, validate, and share an analysis-ready version of one of the few existing long-term monitoring datasets for wild bees in the United States. Since 1999, the Native Bee Inventory and Monitoring Lab (BIML) of the United States Geological Survey has sampled wild-bee communities in the Mid-Atlantic U.

Pollen Protein: Lipid Macronutrient Ratios May Guide Broad Patterns of Bee Species Floral Preferences.

Pollinator nutritional ecology provides insights into plant-pollinator interactions, coevolution, and the restoration of declining pollinator populations. Bees obtain their protein and lipid nutrient intake from pollen, which is essential for larval growth and development as well as adult health and reproduction. Our previous research revealed that pollen protein to lipid ratios (P:L) shape bumble bee foraging preferences among pollen host-plant species, and these preferred ratios link to bumble bee colony health and fitness.

Cytokinins Are Abundant and Widespread Among Insect Species.

Cytokinins (CKs) are a class of compounds that have long been thought to be exclusively plant growth regulators. Interestingly, some species of phytopathogenic bacteria and fungi have been shown to, and gall-inducing insects have been hypothesized to, produce CKs and use them to manipulate their host plants. We used high performance liquid chromatography-electrospray ionization tandem mass spectrometry (HPLC-MS/MS) to examine concentrations of a wide range of CKs in 17 species of phytophagous insects, including gall- and non-gall-inducing species from all six orders of Insecta that contain species known to induce galls: Thysanoptera, Hemiptera, Lepidoptera, Coleoptera, Diptera, and Hymenoptera.

Balancing Disturbance and Conservation in Agroecosystems to Improve Biological Control.

Disturbances associated with agricultural intensification reduce our ability to achieve sustainable crop production. These disturbances stem from crop-management tactics and can leave crop fields more vulnerable to insect outbreaks, in part because natural-enemy communities often tend to be more susceptible to disturbance than herbivorous pests. Recent research has explored practices that conserve natural-enemy communities and reduce pest outbreaks, revealing that different components of agroecosystems can influence natural-enemy populations.