Publications by authors named "John T Stanfield"

Cultures of three Listeria monocytogenes serotypes and three Salmonella spp. were applied to the exterior surfaces of waxed cardboard or plastic milk containers. Contamination sites were sampled with premoistened cotton swabs during 14 d of refrigeration.

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A strain of Yersinia enterocolitica isolated from a patient in a milk-associated yersiniosis outbreak in Tennessee, Mississippi and Arkansas in the summer of 1982 was used to contaminate the exterior of refrigerated milk containers. The bacteria survived on the containers for as long as 21 d, as shown by recovery on MacConkey agar plates or in veal infusion broth. Y.

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An experimental suckling mouse intraperitoneal injection test was compared with four plasmid-associated tests (adult mouse peroral exposure, adult mouse intraperitoneal injection, auto-agglutination and plasmid detection by gel electrophoresis) to measure Yersinia enterocolitica pathogenicity. Of eight Vwa plasmid-harboring strains (O:3; O:4,32; O:5,27; O:8; O:9; O:13; O:21; and O:Tacoma) and one isogenic plasmidless strain (O:8), all Vwa plasmid-harboring strains gave identical results in all tests except the two adult mouse tests. In studies of 35 clinical strains of Y.

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Article Synopsis
  • From December 1981 to February 1982, 87 people in Seattle contracted yersiniosis linked to contaminated tofu containing Yersinia enterocolitica.
  • Two distinct strains, 0:8 and 0:Tacoma, were identified among patients, while similar strains were also found at the tofu manufacturing site.
  • The investigation suggested that water used in tofu processing was the likely source of infection, although the origin of the 0:Tacoma strain remains unknown.
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