Transplantation of human stem cell-derived hepatocytes in an animal model of acute liver failure.

Introduction: Hepatocyte cell transplantation can be life-saving in patients with acute liver failure (ALF); however, primary human hepatocyte transplantation is limited by the scarcity of donor hepatocytes. We investigated the effect of stem cell-derived, hepatocyte-like cells in an animal xenotransplant model of ALF.

Methods: Intraperitoneal d-galactosamine was used to develop a lethal model of ALF in the rat.