Electrophoresis of a small sphere in a cylinder of finite length.

The pressure due to electrophoretic motion of a charged colloidal sphere in a fluid-filled circular cylinder is determined in the limit in which the sphere radius is small compared with that of the cylinder. If the ends of the cylinder are open, pressure-driven Poiseuille flow occurs, but the magnitude of this flow is shown to be small when the cylinder is long compared to its radius. It is concluded that the flow has little effect upon electrophoretic velocities, unlike when the diameter of the sphere is comparable to that of the cylinder in which case the Poiseuille flow increases electrophoretic velocities and creates long-range interactions between spheres.

The 22q11.2 region regulates presynaptic gene-products linked to schizophrenia.

It is unclear how the 22q11.2 deletion predisposes to psychiatric disease. To study this, we generated induced pluripotent stem cells from deletion carriers and controls and utilized CRISPR/Cas9 to introduce the heterozygous deletion into a control cell line.

The lateral electric force on an off-axis sphere in a circular cylinder.

The electrophoretic velocity of a sphere within a liquid-filled circular cylinder in a direction parallel to the cylinder axis has been studied by Yariv and Brenner (Phys. Fluids 2002, 14, 3354-3357; SIAM J. Appl.

Exploration of prescribing error reporting across primary care: a qualitative study.

Objectives: To explore barriers and facilitators to prescribing error reporting across primary care.

Design: Qualitative semi-structured face-to-face and telephone interviews were conducted to explore facilitators and barriers to reporting prescribing errors. Data collection and thematic analysis were informed by the COM-B model of behaviour change.

Clinical Significance of Plasma CD9-Positive Exosomes in HIV Seronegative and Seropositive Lung Cancer Patients.

Recently, the role of exosomes in the progression of both cancer and HIV (human immunodeficiency virus) has been described. This study investigates the clinical significance of CD9-positive plasma exosomes in lung cancer patients, healthy individuals, and HIV-positive patients with or without lung cancer. Using a verified with transmission electron microscopy double-sandwich ELISA technique, plasma-derived exosomes were isolated and quantified from 210 lung cancer patients (including 44 metastatic patients with progressive disease after chemotherapy), 49 healthy controls, 20 patients with pulmonary granulomas, 19 HIV+ patients with lung cancer, 31 HIV+ patients without cancer, and 3 HIV+ patients with pulmonary granulomas.

is a non-lethal modifier of the mosaic eye phenotype in .

Genetic screens have been used to identify genes involved in the regulation of different biological processes. We identified growth mutants in a Flp/FRT screen using the eye to identify conditional regulators of cell growth and cell division. One mutant identified from this screen, , was mapped and characterized by researchers in undergraduate genetics labs as part of the Fly-CURE.

Prescribing error reporting in primary care: a narrative synthesis systematic review.

Prescribing errors can cause avoidable harm to patients. Most prescriptions originate in primary care, where medications tend to be self-administered and errors have the most potential to cause harm. Reporting prescribing errors can identify trends and reduce the risk of the reoccurrence of incidents; however, under-reporting is common.

Complete Genome Sequences of Cluster G Mycobacteriophage Darionha, Cluster A Mycobacteriophage Salz, and Cluster J Mycobacteriophage ThreeRngTarjay.

Mycobacteriophages Darionha, Salz, and ThreeRngTarjay are mycobacteriophages isolated using the host mc155. Following isolation from soil samples, all three siphoviridae phages were characterized, and their genomes were sequenced and annotated.

Internet-based pharmacy and centralised dispensing: an exploratory mixed-methods study of the views of family practice staff.

Objectives: Over the last decade, technological advances, market competition and increasing pressures for efficiencies across healthcare systems have resulted in changes to the processes and policies involved in medicines prescribing and dispensing. The aim of this study was to explore the views of family practice staff, including GPs, on the perceived impact of changes associated with remote dispensing and the increasing availability of distance-selling pharmacies.

Methods: Exploratory mixed-methods study using qualitative focus groups and an online cross-sectional survey distributed to a non-probability sample of staff from family practices across England.

Solid-state nanopore hydrodynamics and transport.

The resistive pulse method based on measuring the ion current trace as a biomolecule passing through a nanopore has become an important tool in biotechnology for characterizing molecules. A detailed physical understanding of the translocation process is essential if one is to extract the relevant molecular properties from the current signal. In this Perspective, we review some recent progress in our understanding of hydrodynamic flow and transport through nanometer sized pores.

Repetitive head injury in adolescent mice: A role for vascular inflammation.

Repetitive mild traumatic brain injury during adolescence can induce neurological dysfunction through undefined mechanisms. Interleukin-1 (IL-1) contributes to experimental adult diffuse and contusion TBI models, and IL-1 antagonists have entered clinical trials for severe TBI in adults; however, no such data exist for adolescent TBI. We developed an adolescent mouse repetitive closed head injury (rCHI) model to test the role of IL-1 family members in post-injury neurological outcome.

Combining NGN2 Programming with Developmental Patterning Generates Human Excitatory Neurons with NMDAR-Mediated Synaptic Transmission.

Transcription factor programming of pluripotent stem cells (PSCs) has emerged as an approach to generate human neurons for disease modeling. However, programming schemes produce a variety of cell types, and those neurons that are made often retain an immature phenotype, which limits their utility in modeling neuronal processes, including synaptic transmission. We report that combining NGN2 programming with SMAD and WNT inhibition generates human patterned induced neurons (hpiNs).

Interleukin-1 Receptor 1 Deletion in Focal and Diffuse Experimental Traumatic Brain Injury in Mice.

Important differences in the biology of focal and diffuse traumatic brain injury (TBI) subtypes may result in unique pathophysiological responses to shared molecular mechanisms. Interleukin-1 (IL-1) signaling has been tested as a potential therapeutic target in preclinical models of cerebral contusion and diffuse TBI, and in a phase II clinical trial, but no published studies have examined IL-1 signaling in an impact/acceleration closed head injury (CHI) model. We hypothesized that genetic deletion of IL-1 receptor-1 (IL-1R1 KO) would be beneficial in focal (contusion) and CHI in mice.

Complete Genome Sequences of Cluster A Mycobacteriophages BobSwaget, Fred313, KADY, Lokk, MyraDee, Stagni, and StepMih.

Seven mycobacteriophages from distinct geographical locations were isolated, using mc155 as the host, and then purified and sequenced. All of the genomes are related to cluster A mycobacteriophages, BobSwaget and Lokk in subcluster A2; Fred313, KADY, Stagni, and StepMih in subcluster A3; and MyraDee in subcluster A18, the first phage to be assigned to that subcluster.

Cell diversity and network dynamics in photosensitive human brain organoids.

In vitro models of the developing brain such as three-dimensional brain organoids offer an unprecedented opportunity to study aspects of human brain development and disease. However, the cells generated within organoids and the extent to which they recapitulate the regional complexity, cellular diversity and circuit functionality of the brain remain undefined. Here we analyse gene expression in over 80,000 individual cells isolated from 31 human brain organoids.

Astrocytic IP Rs: Contribution to Ca signalling and hippocampal LTP.

Astrocytes regulate hippocampal synaptic plasticity by the Ca dependent release of the N-methyl d-aspartate receptor (NMDAR) co-agonist d-serine. Previous evidence indicated that d-serine release would be regulated by the intracellular Ca release channel IP receptor (IP R), however, genetic deletion of IP R2, the putative astrocytic IP R subtype, had no impact on synaptic plasticity or transmission. Although IP R2 is widely believed to be the only functional IP R in astrocytes, three IP R subtypes (1, 2, and 3) have been identified in vertebrates.

Repulsion Between Finite Charged Plates with Strongly Overlapped Electric Double Layers.

Screened Coulomb interactions between uniformly charged flat plates are considered at very small plate separations for which the Debye layers are strongly overlapped, in the limit of small electrical potentials. If the plates are of infinite length, the disjoining pressure between the plates decays as an inverse power of the plate separation. If the plates are of finite length, we show that screening Debye layer charges close to the edge of the plates are no longer constrained to stay between the plates, but instead spill out into the surrounding electrolyte.

Mechanical properties of β-HMX.

Background: For a full understanding of the mechanical properties of a material, it is essential to understand the defect structures and associated properties and microhardness indentation is a technique that can aid this understanding.

Results: The Vickers hardness on (010), {011} and {110} faces lay in the range of 304-363 MPa. The Knoop Hardnesses on the same faces lay in the range 314-482 MPa.

Growth and dislocation studies of β-HMX.

Background: The defect structure of organic materials is important as it plays a major role in their crystal growth properties. It also can play a subcritical role in "hot-spot" detonation processes of energetics and one such energetic is cyclotetramethylene-tetranitramine, in the commonly used beta form (β-HMX).

Results: The as-grown crystals grown by evaporation from acetone show prismatic, tabular and columnar habits, all with {011}, {110}, (010) and (101) faces.

Ergosterol, an orphan fungal microbe-associated molecular pattern (MAMP).

Fungal pathogens continue to pose a significant threat to crop production and food supply. The early stages of plant-fungus interactions are mostly mediated by microbe-associated molecular pattern (MAMP) molecules, perceived by plant pattern recognition receptors (PRRs). Currently, the identified fungal MAMP molecules include chitin, chitosan, β-glucans, elicitins and ergosterol.

Differences in kainate receptor involvement in hippocampal mossy fibre long-term potentiation depending on slice orientation.

Long-term potentiation (LTP) is a well-established experimental model used to investigate the synaptic basis of learning and memory. LTP at mossy fibre - CA3 synapses in the hippocampus is unusual because it is normally N-methyl-d-aspartate (NMDA) receptor-independent. Instead it seems that the trigger for mossy fibre LTP involves kainate receptors (KARs).

Airway spray cryotherapy: initial outcomes from a multiinstitutional registry.

Background: Spray cryotherapy (SCT) uses a noncontact system to deliver liquid nitrogen (2 to 4 psi) through an endoscopic catheter. Rapid freezing and thawing of tissue causes cellular death and is also hemostatic. We report the preliminary results from 6 institutions in which SCT was used for the treatment of malignant airway tumors.

Strong enhancement of streaming current power by application of two phase flow.

We show that the performance of a streaming-potential based microfluidic energy conversion system can be strongly enhanced by the use of two phase flow. Injection of gas bubbles into a liquid-filled channel increases both the maximum output power and the energy conversion efficiency. In single-phase systems the internal conduction current induced by the streaming potential limits the output power, whereas in a two-phase system the bubbles reduce this current and increase the power.

The methylazoxymethanol acetate (MAM-E17) rat model: molecular and functional effects in the hippocampus.

Administration of the DNA-alkylating agent methylazoxymethanol acetate (MAM) on embryonic day 17 (E17) produces behavioral and anatomical brain abnormalities, which model some aspects of schizophrenia. This has lead to the premise that MAM rats are a neurodevelopmental model for schizophrenia. However, the underlying molecular pathways affected in this model have not been elucidated.