A real-time PCR antibiogram for drug-resistant sepsis.

Current molecular diagnostic techniques for susceptibility testing of septicemia rely on genotyping for the presence of known resistance cassettes. This technique is intrinsically vulnerable due to the inability to detect newly emergent resistance genes. Traditional phenotypic susceptibility testing has always been a superior method to assay for resistance; however, relying on the multi-day growth period to determine which antimicrobial to administer jeopardizes patient survival.

Engineered approaches to the stem cell microenvironment for cardiac tissue regeneration.

Micro- and nanoscale engineering approaches in medicine have the potential to recreate physiologically relevant stem cell microenvironments to enhance our understanding of stem cell behaviour and bring stem cell therapy closer to fruition. The realization of such advancements will impact a number of therapeutic applications, the most immediate of which may be the repair of heart tissue. Despite profound advances in creating physiologically relevant in vivo stem cell niches through the control of biochemical regulatory factors, further synergism of innovative techniques promise to elucidate the impact of a number of physical cues such as stem cell differentiation into cardiac cells, the electromechanical coupling among these cells, and the formation of bioengineered cardiac tissue grafts.

Disassembly of a core-satellite nanoassembled substrate for colorimetric biomolecular detection.

The disassembly of a core-satellite nanostructured substrate is presented as a colorimetric biosensor observable under dark-field illumination. The fabrication method described herein utilizes thiol-mediated adsorption and streptavidin-biotin binding to self-assemble core-satellite nanostructures with a sacrificial linking peptide. Biosensing functionality is demonstrated with the protease trypsin, and the optical properties of the nanoassemblies are characterized.

Strategies for nanoplasmonic core-satellite biomolecular sensors: Theory-based Design.

We present a systematic theoretical study of core-satellite gold nanoparticle assemblies using the Generalized Multiparticle Mie formalism. We consider the importance of satellite number, satellite radius, the core radius, and the satellite distance, and we present approaches to optimize spectral shift due to satellite attachment or release. This provides clear strategies for improving the sensitivity and signal-to-noise ratio for molecular detection, enabling simple colorimetric assays.

Hydrodynamically tunable optofluidic cylindrical microlens.

In this work, we report the design, fabrication, and characterization of a tunable optofluidic microlens that focuses light within a microfluidic device. The microlens is generated by the interface of two co-injected miscible fluids of different refractive indices, a 5 M CaCl(2) solution (n(D) = 1.445) and deionized (DI) water (n(D) = 1.

"Microfluidic drifting"--implementing three-dimensional hydrodynamic focusing with a single-layer planar microfluidic device.

We introduce a novel fluid manipulation technique named "microfluidic drifting" to enable three-dimensional (3D) hydrodynamic focusing with a simple single-layer planar microfluidic device.