Objective: To determine whether altering selected components of sequential culture media can improve early development variables of human embryos.
Design: Prospective, randomized, sibling oocyte split trial.
Setting: Private ART center.
J Assist Reprod Genet
August 2002
Purpose: To assess the ability of various facets of embryo culture (microscope stage warmers, volumes of culture media, culture vessel lids, and type of culture incubator) to maintain a constant temperature in vitro.
Methods: Ability to maintain 37.0 degrees C in the microenvironment of gametes was recorded by digital thermocouple in the chosen facets of in vitro culture.