Publications by authors named "John P Gotham"
Electron Paramagnetic Resonance (EPR) spectroscopy coupled with spin traps/probes enables quantitative determination of reactive nitrogen and oxygen species (RNOS). Even with numerous studies using spin probes, the methodology has not been rigorously investigated. The autoxidation of spin probes has been commonly overlooked.
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- * The study successfully utilized the CRISPR-Cas9 system to target and induce specific mutations in the ndr2 gene in zebrafish embryos, leading to the expected cyclopic phenotypes.
- * Analysis revealed that most induced mutations resulted in small changes that disrupted normal gene function, enhancing the understanding of ndr2's role and demonstrating the effectiveness of CRISPR technology for targeted genetic studies.