Publications by authors named "John Nishikawa"
Article Synopsis
- The study presents the first complete genomic sequence of human parainfluenza virus 4 (HPIV4), a virus whose genome was previously incomplete.
- The genomic sequence was obtained from a clinical isolate called SKPIV4 at a hospital in Toronto and shows high similarity (95%-97%) with existing partial sequences for HPIV4B.
- HPIV4 has the longest genome in the Rubulavirus genus at over 17 kb, providing valuable new information that will help further research on this respiratory virus.
A long RT-PCR method was developed to amplify the norovirus genome. Starting from RNA extracted directly from clinical samples and using broadly reactive primers, it can generate near full-length amplicons that allow for easy determination of the near complete genomic sequence. Two norovirus isolates from Toronto, Canada, in 2002 and 2005 were sequenced.
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