3' terminal nucleotides determine thermodynamic stabilities of mismatches at the ends of RNA helices.

The thermodynamic stabilities of consecutive mismatches at the ends of RNA helices are determined by the 3' terminal nucleotides. More than 40 RNA duplexes containing terminal motifs of 3 or more nucleotides were studied by optical melting experiments. Up to three noncanonical pairs of nucleotides at the end of RNA helices provide additional thermodynamic stability.