Inertial motion on the earth's spheroidal surface.

As seen by an observer in the rotating frame, the earth's small spheroidal deformations neutralize the centrifugal force, leaving only the smaller Coriolis force to govern the "inertial" motion of objects that move on its surface, assumed smooth and frictionless. Previous studies of inertial motion employ weakly spheroidal equations of motion that ignore the influence of the centrifugal force and yet treat the earth as a sphere. The latitude dependence of these equations renders them strongly nonlinear.

A Web-based Educational Intervention to Increase Perianesthesia Nurses' Knowledge, Attitude, and Intention to Promote Safe Use, Storage, and Disposal of Opioids.

Purpose: The purpose of this study was to determine if a web-based educational intervention increased knowledge, attitudes, and intention of perianesthesia nurses regarding opioid discharge education (including safe use, storage, and disposal of opioids). Secondary outcomes were to determine Perceived Behavioral Control, subjective norms, and familiarity with American Society of PeriAnesthesia Nurses (ASPAN) guidance on opioid education.

Design: A pre-test, post-test longitudinal design.

Compliance With Opioid Disposal Following Opioid Disposal Education in Surgical Patients: A Systematic Review.

Purpose: The present opioid epidemic in the United States is a significant cause for concern in healthcare. In 1995, the concept of pain was introduced as the fifth vital sign. Since then, the sales of opioids have increased dramatically, as have the number of opioid deaths.

Surgical Patient Compliance With Healthcare Facility-Provided In-home Opioid Disposal Products: A Systematic Review.

Objective: The aim of this study was to review the literature regarding the use of an in-home opioid disposal product on unused opioids after surgery.

Background: The opioid epidemic in the United States is a major cause of concern for healthcare facilities. The misuse and diversion of retained opioids after a surgical procedure continues to contribute to this problem.

The Role of a Certified Registered Nurse Anesthetist Led Acute Pain Service in Preventing Persistent Postoperative Opioid Use.

Given the present opioid crisis, the use of opioids in the hospital setting is an increasing concern among hospital administrators and healthcare professionals. A serious problem related to surgical care is persistent postoperative opioid use among previously opioid-naïve patients. Certified registered nurse anesthetists (CRNAs) are strategically positioned within the hospital setting to address these concerns.

Periodic bouncing modes for two uniformly magnetized spheres. I. Trajectories.

We consider a uniformly magnetized sphere that moves without friction in a plane in response to the field of a second, identical, fixed sphere, making elastic hard-sphere collisions with this sphere. We seek periodic solutions to the associated nonlinear equations of motion. We find closed-form mathematical solutions for small-amplitude modes and use these to characterize and validate our large-amplitude modes, which we find numerically.

Periodic bouncing modes for two uniformly magnetized spheres. II. Scaling.

A uniformly magnetized sphere moves without friction in a plane in response to the field of a second, identical, fixed sphere and makes elastic hard-sphere collisions with this sphere. Numerical simulations of the threshold energies and periods of periodic finite-amplitude nonlinear bouncing modes agree with small-amplitude closed-form mathematical results, which are used to identify scaling parameters that govern the entire amplitude range, including power-law scaling at large amplitudes. Scaling parameters are combinations of the bouncing number, the rocking number, the phase, and numerical factors.

Use of F Differential Labelling for the Simultaneous Detection and Monitoring of Three Individual Proteins in a Serum Environment.

Protein behavior in complex mixtures, such as biological fluids, is often modeled by simplified buffer systems in solution. Here we have used the recently described differential F labelling approach (with NMR detection) to monitor and compare the solution behaviour of three proteins at once: human serum albumin (HSA), transferrin (TrF), and immunoglobulin G (IgG), both in serum and in buffer. We demonstrate that monitoring three proteins simultaneously and independently in biological fluid is possible, and that the presence of other endogenous components greatly changes the association characteristics of these proteins.

F Dark-State Exchange Saturation Transfer NMR Reveals Reversible Formation of Protein-Specific Large Clusters in High-Concentration Protein Mixtures.

Proteins frequently exist as high-concentration mixtures, both in biological environments and increasingly in biopharmaceutical co-formulations. Such crowded conditions promote protein-protein interactions, potentially leading to formation of protein clusters, aggregation, and phase separation. Characterizing these interactions and processes in situ in high-concentration mixtures is challenging due to the complexity and heterogeneity of such systems.

Publisher Correction: Critical Southern Ocean climate model biases traced to atmospheric model cloud errors.

'In the original HTML version of this Article, ref.12 was incorrectly cited in the first sentence of the first paragraph of the Introduction. The correct citation is ref.

Critical Southern Ocean climate model biases traced to atmospheric model cloud errors.

The Southern Ocean is a pivotal component of the global climate system yet it is poorly represented in climate models, with significant biases in upper-ocean temperatures, clouds and winds. Combining Atmospheric and Coupled Model Inter-comparison Project (AMIP5/CMIP5) simulations, with observations and equilibrium heat budget theory, we show that across the CMIP5 ensemble variations in sea surface temperature biases in the 40-60°S Southern Ocean are primarily caused by AMIP5 atmospheric model net surface flux bias variations, linked to cloud-related short-wave errors. Equilibration of the biases involves local coupled sea surface temperature bias feedbacks onto the surface heat flux components.

F NMR as a Tool for Monitoring Individual Differentially Labeled Proteins in Complex Mixtures.

The ability to monitor the behavior of individual proteins in complex mixtures has many potential uses, ranging from analysis of protein interactions in highly concentrated solutions, modeling biological fluids or the intracellular environment, to optimizing biopharmaceutical co-formulations. Differential labeling NMR approaches, which traditionally use N or C isotope incorporation during recombinant expression, are not always practical in cases when endogenous proteins are obtained from an organism, or where the expression system does not allow for efficient labeling, especially for larger proteins. This study proposes differential labeling of proteins by covalent attachment of F groups with distinct chemical shifts, giving each protein a unique spectral signature which can be monitored by F NMR without signal overlap, even in complex mixtures, and without any interfering signals from the buffer or other unlabeled components.

Evaluation of satellite and reanalysis-based global net surface energy flux and uncertainty estimates.

The net surface energy flux is central to the climate system yet observational limitations lead to substantial uncertainty. A combination of satellite-derived radiative fluxes at the top of atmosphere adjusted using the latest estimation of the net heat uptake of the Earth system, and the atmospheric energy tendencies and transports from the ERA-Interim reanalysis are used to estimate surface energy flux globally. To consider snowmelt and improve regional realism, land surface fluxes are adjusted through a simple energy balance approach at each grid point.

Periodic nonlinear sliding modes for two uniformly magnetized spheres.

A uniformly magnetized sphere slides without friction along the surface of a second, identical sphere that is held fixed in space, subject to the magnetic force and torque of the fixed sphere and the normal force. The free sphere has two stable equilibrium positions and two unstable equilibrium positions. Two small-amplitude oscillatory modes describe the sliding motion of the free sphere near each stable equilibrium, and an unstable oscillatory mode describes the motion near each unstable equilibrium.

An internal thioester in a pathogen surface protein mediates covalent host binding.

To cause disease and persist in a host, pathogenic and commensal microbes must adhere to tissues. Colonization and infection depend on specific molecular interactions at the host-microbe interface that involve microbial surface proteins, or adhesins. To date, adhesins are only known to bind to host receptors non-covalently.

Structural insights into the targeting of mRNA GU-rich elements by the three RRMs of CELF1.

The CUG-BP, Elav-like family (CELF) of RNA-binding proteins control gene expression at a number of different levels by regulating pre-mRNA splicing, deadenylation and mRNA stability. We present structural insights into the binding selectivity of CELF member 1 (CELF1) for GU-rich mRNA target sequences of the general form 5'-UGUNxUGUNyUGU and identify a high affinity interaction (Kd ∼ 100 nM for x = 2 and y = 4) with simultaneous binding of all three RNA recognition motifs within a single 15-nt binding element. RNA substrates spin-labelled at either the 3' or 5' terminus result in differential nuclear magnetic resonance paramagnetic relaxation enhancement effects, which are consistent with a non-sequential 2-1-3 arrangement of the three RNA recognition motifs on UGU sites in a 5' to 3' orientation along the RNA target.

Saturation transfer difference NMR reveals functionally essential kinetic differences for a sugar-binding repressor protein.

The binding kinetics of disaccharides trehalose and trehalose-6-phosphate to repressor protein TreR have been determined using STD NMR and shed light on the contrasting biological roles of these two sugars.

Electroosmotic flow in vapor deposited silicon dioxide and nitride microchannels.

Electroosmotic flow was studied in thin film microchannels with silicon dioxide and silicon nitride sidewalls formed using plasma-enhanced chemical vapor deposition (PECVD). A sacrificial etching process was employed for channel fabrication allowing for cross-sections with heights of 3 mum, ranging from 2 mum to 50 mum in width. Flow rates were measured for single channels and multichannel electroosmotic pump structures for pH levels ranging from 2.

Thin film electro-osmotic pumps for biomicrofluidic applications.

Electro-osmotic flow (EOF) pumps are attractive for fluid manipulation in microfluidic channels. Open channel EOF pumps can produce high pressures and flow rates, and are relatively easy to fabricate on-chip or integrate with other microfluidic or electrical components. An EOF pump design that is conducive to on-chip fabrication consists of multiple small channel arms feeding into a larger flow channel.

The anterior cardiac plexus: an intrinsic neurosecretory site within the stomatogastric nervous system of the crab Cancer productus.

The stomatogastric nervous system (STNS) of decapod crustaceans is modulated by both locally released and circulating substances. In some species, including chelate lobsters and freshwater crayfish, the release zones for hormones are located both intrinsically to and at some distance from the STNS. In other crustaceans, including Brachyuran crabs, the existence of extrinsic sites is well documented.

Immunocytochemical evidence for nitric oxide- and carbon monoxide-producing neurons in the stomatogastric nervous system of the crayfish Cherax quadricarinatus.

Nitric oxide (NO) and carbon monoxide (CO) have been shown to serve neuromodulatory roles in both vertebrates and invertebrates. Here, we use antibodies to their respective biosynthetic enzymes, nitric oxide synthase (NOS) and heme oxygenase 2 (HO-2), to map the distribution of putative gas-producing neurons in the stomatogastric nervous system (STNS) of the crayfish Cherax quadricarinatus. In this species, NOS immunolabeling is found in the neuropil of the stomatogastric ganglion (STG).