Publications by authors named "John Linney"
Article Synopsis
- * Research on Corynebacterium glutamicum reveals that resveratrol accumulates in the cell membrane and wall, which leads to membrane rigidity and affects cell viability.
- * Supplementing with certain fatty acids, specifically palmitelaidic and linoleic acid, can reduce the toxic effects of resveratrol, resulting in a three-fold increase in production, suggesting this strategy could be applied to other bioproducts as well.
Identification of membrane engineering targets for increased butanol tolerance in Clostridium saccharoperbutylacetonicum.
Biochim Biophys Acta Biomembr
December 2023
Article Synopsis
- Microbial cell factories are increasingly being explored to produce butanol, an eco-friendly solvent and biofuel alternative to fossil fuels.
- Solventogenic Clostridia, particularly Clostridium saccharoperbutylacetonicum N1-4, are favored for their natural ability to synthesize butanol, though high toxicity limits large-scale application.
- This study reveals how butanol affects cell membranes, particularly the increase of phosphatidylglycerol lipids, suggesting that modifying membrane properties could enhance butanol tolerance and improve production efficiency.
Article Synopsis
- * Using a modified synthetic must for adaptation, the researchers found that the adapted yeast strain H14A7-etoh showed increased resistance to sulfite but only slight improvement in ethanol tolerance, alongside a reduced ability to ferment sugars.
- * Genetic analysis revealed instability in the hybrid's genome, with different adaptations across its subgenomes, including duplicated chromosomes and changes in gene expression affecting lipid composition related to stress tolerance mechanisms.
Biological membranes form the boundaries to cells. They are integral to cellular function, retaining the valuable components inside and preventing access of unwanted molecules. Many different classes of molecules demonstrate disruptive properties to the plasma membrane.
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