Publications by authors named "John J Tomes"
We report on shear-ordered polymer photonic crystals demonstrating intense structural color with a photonic bandgap at 270 nm. Our work examines this UV structural color, originating from a low refractive index contrast polymer composite system as a function of the viewing angle. We report extensive characterization of the angle-dependent nature of this color in the form of 'scattering cones', which showed strong reflectivity in the 275-315 nm range.
View Article and Find Full Text PDFis a neglected gastro-intestinal tapeworm, commonly infecting horses worldwide. Molecular investigation of is hampered by a lack of tools to better understand the host-parasite interface. This interface is likely influenced by parasite derived immune modulators released in the secretome as free proteins or components of extracellular vesicles (EVs).
View Article and Find Full Text PDFA three-dimensional goniometric study of thin-film polymer photonic crystals investigates how the chromaticity of structural color is correlated to structural ordering. Characterization of chromaticity and the angular properties of structural color are presented in terms of CIE 1931 color spaces. We examine the viewing angle dependency of the Bragg scattering cone relative to sample symmetry planes, and our results demonstrate how increased ordering influences angular scattering width and anisotropy.
View Article and Find Full Text PDFHelminths like release excretory/secretory (E/S) products that modulate host immunity to enable infection. Extracellular vesicles (EVs) are among these E/S products, yet molecular mechanisms and functionality of EV interaction with host immune cells is unknown. Here we demonstrate that EVs released by schistosomula are internalised by human monocyte-derived dendritic cells (moDCs).
View Article and Find Full Text PDFArticle Synopsis
- - The study investigates the effectiveness of two methods for isolating extracellular vesicles (EVs) from the helminth pathogen Fasciola hepatica, comparing differential centrifugation (DC) and size exclusion chromatography (SEC).
- - Results showed that SEC produced EVs that were smaller, purer, and had less contamination from excretory-secretory proteins (ESPs) compared to those purified through DC, which retained more diverse proteins.
- - The findings indicate that SEC is a superior method for EV purification and suggest it should be the preferred choice for future studies involving EVs to avoid issues with contamination.