Analysis of Range and Use of a Hybrid Hydrogen Peroxide System for Biosafety Level 3 and Animal Biosafety Level 3 Agriculture Laboratory Decontamination.

Introduction: The applications of fumigation and the challenges that high-containment facilities face in achieving effective large volume decontamination are well understood. The Biosecurity Research Institute at Kansas State University sought to evaluate a novel system within their biosafety level 3 (BSL-3) and animal biosafety level 3 agriculture (ABSL-3Ag) facility.

Methods: The system chosen for this study is the CURIS Hybrid Hydrogen Peroxide (HHP) system, comprising a mobile 36-pound (16 kg) device delivering a proprietary 7% hydrogen peroxide (HO) solution.

Hydrogen Peroxide Methods for Decontaminating N95 Filtering Facepiece Respirators.

During a pandemic, when the supply of N95 filtering facepiece respirators (FFRs) is limited, FFRs may be decontaminated by methods that inactivate pathogens as long as they do not damage FFR function. Hydrogen peroxide (HO) is widely used for decontamination in medical settings. To review the literature on the use of HO to decontaminate N95 FFRs and identify methods that inactivate virus and preserve FFR filtration efficiency and fit.

Challenges and Solutions With Agricultural Animal High Containment Waste Disposal.

Waste disposal in Agricultural Animal High Containment Animal Biosafety Level 3Ag and Animal Biosafety Level 4Ag (ABSL-3Ag and ABSL-4Ag) research facilities necessitates significantly more attention to detail in operations than that required in lower-containment-level laboratories. The unique features and requirements of agricultural-related research involve additional equipment and systems to safely transfer decontaminated waste out of the facility. The waste stream coming from ABSL-3Ag and ABSL-4Ag facilities, or high containment agricultural research waste, consists of many forms and differs from most research facility waste in that it is produced from research with livestock or other species loose housed, with the animal room serving as primary containment.

DNA adducts and liver DNA replication in rats during chronic exposure to N-nitrosodimethylamine (NDMA) and their relationships to the dose-dependence of NDMA hepatocarcinogenesis.

Exposure of rats to the hepatocarcinogen N-nitrosodimethylamine (NDMA) (0.2-2.64 ppm in the drinking water) for up to 180 days resulted in rapid accumulation of N7- and O6-methylguanine in liver and white blood cell DNA, maximum adduct levels being reached within 1-7 days, depending on the dose.