Biodiversity impacts of the 2019-2020 Australian megafires.

With large wildfires becoming more frequent, we must rapidly learn how megafires impact biodiversity to prioritize mitigation and improve policy. A key challenge is to discover how interactions among fire-regime components, drought and land tenure shape wildfire impacts. The globally unprecedented 2019-2020 Australian megafires burnt more than 10 million hectares, prompting major investment in biodiversity monitoring.

Meal or mate: Exploring the evidence of sexual cannibalism among amphibians.

Active forms of cannibalism that involve predation of live conspecifics occur widely among amphibians, most notably by tadpoles that feed on each other and adults that feed on juveniles. In contrast, cannibalism among amphibian adults (adult-adult cannibalism) is less often reported and there have been no investigations on the occurrence of sexual cannibalism in this group to date. In this study, we present an observation of potential sexual cannibalism involving an adult female green and golden bell frog, , preying on a conspecific adult male during the species' breeding season.

It's not easy being green: Comparing typical skin colouration among amphibians with colour abnormalities associated with chromatophore deficits.

Amphibians can obtain their colour from a combination of several different pigment and light reflecting cell types called chromatophores, with defects in one or several of the cells leading to colour abnormalities. There is a need for better recording of colour abnormalities within wild amphibian populations, as this may provide baseline data that can be used to determine changes in environmental conditions and population dynamics, such as inbreeding. In this study, we provide records of several types of chromatophore deficiencies, including those involving iridophores, xanthophores and melanophores, among two Australian tree frog species; the green and golden bell frog, , and the eastern dwarf tree frog, .

High clutch failure rate due to unpredictable rainfall for an ephemeral pool-breeding frog.

Animals that reproduce in temporary aquatic systems expose their offspring to a heightened risk of desiccation, as they must race to complete development and escape before water levels recede. Adults must therefore synchronise reproduction with the changing availability of water, yet the conditions they experience to trigger such an event may not relate to those offspring face throughout development, potentially leading to clutch failure. The sandpaper frog (Lechriodus fletcheri) breeds in ephemeral pools that dry within days to weeks after rainfall has ceased.

Framing Effects, Social Norm Perception, and Tolerance of Lesbian and Gay Individuals: Experimental Evidence From Slovakia.

Perceptions of social norms can have downstream consequences for attitudes and behaviors, especially when it comes to the acceptance of marginalized groups. While interventions focusing on social norms may boost tolerance, few studies test whether variations in norm communication affect individuals' perceptions. Thus, in this paper, we test the effectiveness of three communicative aspects-valence framing (Experiments 1-3), point of view (Experiment 1), and group centrism (Experiment 3)-in shifting perceptions of social norms.

Using citizen science in the photo-identification of adult individuals of an amphibian based on two facial skin features.

Among amphibians, adults have traditionally been identified in capture-mark-recapture studies using invasive marking techniques with associated ethical, cost and logistical considerations. However, species in this group may be strong candidates for photo-identification based on natural skin features that removes many of these concerns, with this technique opening up opportunities for citizen scientists to be involved in animal monitoring programs. We investigated the feasibility of using citizen science to distinguish between individuals of an Australian anuran (the sandpaper frog, ) based on a visual analysis of their natural skin features.

Enhanced colorectal cancer metastases in the alcohol-injured liver.

Metastatic liver disease is a major cause of mortality in colorectal cancer (CRC) patients. Alcohol consumption is a noted risk factor for secondary cancers yet the role of alcoholic liver disease (ALD) in colorectal liver metastases (CRLM) is not defined. This work evaluated tumor cell colonization in the alcoholic host liver using a novel preclinical model of human CRC liver metastases.

Role of a Limited Transarticular Release in Severe Hallux Valgus Correction.

Background: Hallux valgus (HV) treatment is continuously evolving, and no definitive treatment can be recommended. Osteotomies are the main surgical choice for these deformities, but no clear role for soft tissue procedures is available.

Objective: To perform a retrospective comparison of the radiographic and clinical outcomes of 2 groups of patients with severe HV operated with the same osteotomy technique but differing on the type of lateral release.

Recurrent tarsal tunnel syndrome.

Recurrence of tarsal tunnel syndrome after surgery may be due to inadequate release, lack of understanding or appreciation of the actual anatomy involved, variations in the anatomy of the nerve(s), failure to execute the release properly, bleeding with subsequent scarring, damage to the nerve and branches, persistent hypersensitivity of the nerves, and preexisting intrinsic damage to the nerve. Approaches include more thorough release, use of barrier materials to decrease adherence of the nerve to surrounding tissues to avoid traction neuritis, excisions of neuromas using conduits, and consideration of nerve stimulators and systemic medications to deal with persistent neural pain.

Plate position and angular stability: mechanical comparison in sawbone osteotomy models.

Proximal metatarsal osteotomies are inherently unstable and difficult to fix. The purpose of this study was to compare the mechanical stiffness in sawbone models of medially versus dorsally placed plates and then to compare semilocked versus nonlocked plates in different osteotomy configurations. Thirty sawbone models were constructed, fixed either with titanium self-locking or steel mini-fragment plates.

Proximal oblique sliding closing wedge osteotomy for hallux valgus.

Background: The proximal oblique sliding closing wedge osteotomy (POSCOW) technique was developed to address moderate to severe hallux valgus deformity. We present a retrospective multicenter study to analyze the midterm radiological and clinical outcomes of patients treated with this type of proximal osteotomy fixed with plates.

Materials And Methods: One hundred and forty-four patients (187 feet) were operated on between May 2005 and June 2010 in 2 separate centers.

Use of collagen conduits in management of painful neuromas of the foot and ankle.

Background: Painful neuromas of the foot and ankle frequently pose a treatment dilemma due to persistent pain or recurrence after resection. The purpose of this survey was to evaluate the clinical and functional outcomes in patients in which collagen nerve conduits were used as an adjunct to the resection of a painful neuroma. Our prior experience with vein conduits for this purpose suggested that we might have similar success with the use of these devices.

Pedicle and free flaps for painful nerve.

Treatment of the chronic painful nerve by pedicled or free tissue transfer is a complex surgical procedure, requiring specialized microsurgical training and technique. This procedure is indicated only in patients who have had repeated failure of simpler, conventional procedures. Patients with chronic painful peripheral nerves may be potentially salvaged by external neurolysis and circumferential wrapping of the involved segments of nerve with well-vascularized pedicled or free flaps of fascia, subcutaneous fatty tissue, omentum or muscle, or by the replacement of superficial hypersensitive cutaneous areas and nerves with the same tissues.

The failed tarsal tunnel release.

Failed surgical releases of the tarsal tunnel may be due to numerous causes. Many of the failures are due to lack of appreciation of the involved anatomy or inadequate technique. When an insufficient release is done, a revision simply completes the necessary steps.

Tarsal tunnel syndrome.

Tarsal tunnel syndrome, unlike its similar sounding counterpart in the hand, is a significantly misunderstood clinical entity. Confusion concerning the anatomy involved, the presenting symptomatology, the appropriateness and significance of various diagnostic tests, conservative and surgical management, and, finally, the variability of reported results of surgical intervention attests to the lack of consensus surrounding this condition. The terminology involved in various diagnoses for chronic heel pain is also a hodgepodge of poorly understood entities.

Treatment of fragile X-associated tremor ataxia syndrome (FXTAS) and related neurological problems.

Fragile X-associated tremor/ataxia syndrome (FXTAS) is a progressive neurological disorder that affects older adult carriers, predominantly males, of premutation alleles (55 to 200 CGG repeats) of the fragile X (FMR1) gene. Principal features of FXTAS are intention tremor, ataxia, parkinsonism, cognitive decline, and peripheral neuropathy; ancillary features include, autonomic dysfunction, and psychiatric symptoms of anxiety, depression, and disinhibition. Although controlled trials have not been carried out in individuals with FXTAS, there is a significant amount of anecdotal information regarding various treatment modalities.

Toward the automated generation of genome-scale metabolic networks in the SEED.

Background: Current methods for the automated generation of genome-scale metabolic networks focus on genome annotation and preliminary biochemical reaction network assembly, but do not adequately address the process of identifying and filling gaps in the reaction network, and verifying that the network is suitable for systems level analysis. Thus, current methods are only sufficient for generating draft-quality networks, and refinement of the reaction network is still largely a manual, labor-intensive process.

Results: We have developed a method for generating genome-scale metabolic networks that produces substantially complete reaction networks, suitable for systems level analysis.

Testicular and pituitary inclusion formation in fragile X associated tremor/ataxia syndrome.

Purpose: We describe the medical course, neuropathology and testicular pathology in 2 men who died with fragile X associated tremor/ataxia syndrome. Fragile X associated tremor/ataxia syndrome, which is a recently described, late onset neurodegenerative disorder, affects up to a third of males and occasionally females older than age 50 years who are carriers of premutation alleles (55 to 200 CGG repeats) of the fragile X mental retardation 1 gene FMR1. Clinical manifestations of premutation status are distinct from those of the full mutation, which is the cause of the fragile X syndrome.

Outcome analysis of agility total ankle replacement with prior adjunctive procedures: two to six year followup.

Background: A retrospective case review of 65 agility total ankle replacements (64 patients) was done between April, 1998, and March, 2002. The purpose of this study was to more closely identify factors that may be predictive of a favorable outcome, including a comparison of outcome measures between patients who had preoperative corrective procedures and those patients who did not.

Methods: The outcomes of this series of patients were examined with post-operative Short Form (SF)-36 scores as well as chart and radiographic review.

Technique and results of Achilles tendon detachment and reconstruction for insertional Achilles tendinosis.

Background: Insertional Achilles tendinosis is a clinical entity that commonly occurs with other posterior heel disorders such as retrocalcaneal bursitis, Haglund deformity, intratendinous ossification and pretendinous bursitis. Complete detachment and reconstruction of the Achilles tendon was evaluated as a method of treatment for this condition.

Methods: Seventy-five patients (81 heels) were treated over a 5-year period for chronic insertional Achilles tendinosis.