Publications by authors named "John Gotham"
Club Cell Heme Oxygenase-1 Deletion: Effects in Hyperoxia-Exposed Adult Mice.
Oxid Med Cell Longev
February 2021
Thioredoxin reductase-1 (TXNRD1) inhibition activates nuclear factor (erythroid-derived 2)-like 2 (Nrf2) responses and prevents acute lung injury (ALI). Heme oxygenase-1 (HO-1) induction following TXNRD1 inhibition is Nrf2-dependent in airway epithelial (club) cells . The influence of club cell HO-1 on lung development and lung injury responses is poorly understood.
View Article and Find Full Text PDFElectron Paramagnetic Resonance (EPR) spectroscopy coupled with spin traps/probes enables quantitative determination of reactive nitrogen and oxygen species (RNOS). Even with numerous studies using spin probes, the methodology has not been rigorously investigated. The autoxidation of spin probes has been commonly overlooked.
View Article and Find Full Text PDFAurothioglucose enhances proangiogenic pathway activation in lungs from room air and hyperoxia-exposed newborn mice.
Am J Physiol Lung Cell Mol Physiol
June 2020
Bronchopulmonary dysplasia (BPD), a long-term respiratory morbidity of prematurity, is characterized by attenuated alveolar and vascular development. Supplemental oxygen and immature antioxidant defenses contribute to BPD development. Our group identified thioredoxin reductase-1 (TXNRD1) as a therapeutic target to prevent BPD.
View Article and Find Full Text PDFArticle Synopsis
- * The study successfully utilized the CRISPR-Cas9 system to target and induce specific mutations in the ndr2 gene in zebrafish embryos, leading to the expected cyclopic phenotypes.
- * Analysis revealed that most induced mutations resulted in small changes that disrupted normal gene function, enhancing the understanding of ndr2's role and demonstrating the effectiveness of CRISPR technology for targeted genetic studies.