Publications by authors named "John F Halonski"
A carboxylesterase derived from P1 was immobilized onto an epoxy-activated Sepharose resin non-canonical amino acids. The immobilized enzyme exhibited heightened performance in organic solvents, recyclability, and stability at room temperature for over two years. The incorporation of a non-canonical amino acid afforded a high degree of control over the bioorthogonal immobilization reaction.
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- The article discusses the significance of 1,3-dipolar cycloadditions of azides and alkynes in both biology and chemistry, emphasizing their widespread use.
- It addresses the challenges posed by the need for a copper catalyst in these reactions and presents a solution through the development of a copper-free microwave-assisted process.
- The researchers successfully synthesized various triazoles and applied this new method to bioconjugate a full-length protein using unnatural amino acids, marking a pioneering achievement in microwave-mediated protein chemistry.
The efficient preparation of protein bioconjugates represents a route to novel materials, diagnostics, and therapeutics. We previously reported a novel bioorthogonal Glaser-Hay reaction for the preparation of covalent linkages between proteins and a reaction partner; however, deleterious protein degradation was observed under extended reaction conditions. Herein, we describe the systematic optimization of the reaction to increase coupling efficiency and decrease protein degradation.
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- * Performing this reaction on a solid support helps overcome these selectivity issues, resulting in higher purity and yield of the desired products.
- * The study demonstrates the application of this solid-supported method to synthesize natural products, which were then tested for antibacterial properties, showcasing its practical benefits.