Publications by authors named "John F Bratton"

Microcystin-producing cyanobacterial blooms are a global issue threatening drinking water supplies and recreation on lakes and beaches. Direct measurement of microcystins is the only way to ensure waters have concentrations below guideline concentrations; however, analyzing water for microcystins takes several hours to days to obtain data. We tested LightDeck Diagnostics' bead beater cell lysis and two versions of the quantification system designed to give microcystin concentrations within 20 min and compared it to the standard freeze-thaw cycle lysis method and ELISA quantification.

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Article Synopsis
  • * Two intensive sampling events in Lake Erie in 2018 and 2019 provided 100 and 172 samples respectively, revealing significant variations in microcystin (MC) concentrations, with estimates of total MC mass at 11,513 kg and 30,691 kg.
  • * The study found that the concentration of MC varied considerably over short distances, which complicates predictions based on the MC to chlorophyll ratio; improving data collection frequency and modeling spatial variations are recommended solutions.
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In developed, non-agricultural, unsewered areas, septic systems and fertilizer application to lawns and gardens represent two major sources of nitrogen to coastal groundwater, in addition to atmospheric input. This study was designed to distinguish between these two possible nitrogen sources by analyzing groundwater samples for pharmaceutical residuals, because fertilizers do not contain any of these pharmaceuticals, but domestic wastewater commonly does. In addition, several herbicides and insecticides used in lawn treatment were analyzed as indicators of nitrogen delivery to groundwater from fertilizers.

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