Ubiquitous sialometabolism present among oral fusobacteria.

Fusobacterium nucleatum is a ubiquitous member of the human oral flora and is associated with the development of periodontitis and a variety of other types of polymicrobial infections of the mucosa. In the oral cavity, this species is one of the few that is prevalent in both healthy and diseased subgingival plaque. Using microarray analysis, we examined the transcriptional response of F.

Decellularization method influences early remodeling of an allogenic tissue scaffold.

Extracellular matrix-based biomaterials are currently pursued as an alternative to autologous transplants for the treatment of gingival recession and periodontal disease. These grafts offer improved tissue regeneration without the need for a second operative procedure used in current treatments to remove nonresorbable synthetic biomaterials. However, while decellularization is necessary to minimize the potential immunological impact, it can significantly modify the materials architectural and biochemical properties.

The relationship between receptor for advanced glycation end products expression and the severity of periodontal disease in the gingiva of diabetic and non diabetic periodontitis patients.

Objective: Activation of receptor for advanced glycation end products (RAGE) has been implicated in many chronic diseases, including diabetic complications. In this study we examined the relationship between RAGE expression and the morphological changes seen in the gingiva of diabetic and periodontitis patients.

Design: Gingival biopsies from 15 diabetic patients with periodontitis, 25 non diabetic patients with periodontitis and 10 healthy individuals were collected.

Biomechanical behavior of oral soft tissues.

Background: Little attention has been given to understanding the variation in biomechanical behavior of oral soft tissues, and this represents an obstacle for the development of biomaterials that perform with appropriate biomechanical characteristics. With this as our motivation, a uniaxial mechanical analysis was performed on lingual and buccal aspects of the attached gingiva, alveolar mucosa, and buccal mucosa to gain insight into human tissue performance and site-specific mechanical variation.

Methods: A discrete quantitative mechanical evaluation of each soft tissue region using tensile, dynamic compression, and stress relaxation analysis was conducted to correlate tissue structure with function as assessed histologically.

Cellular interactions and biomechanical properties of a unique vascular-derived scaffold for periodontal tissue regeneration.

These investigations describe the development of a novel ex vivo three-dimensional scaffold derived from the human umbilical vein (HUV), and its potential as a regenerative matrix for tissue regeneration. Unique properties associated with the vascular wall have shown potential to function as a surgical barrier for guided tissue regeneration, particularly with the regeneration of periodontal tissues. HUV was isolated from umbilical cords using a semiautomated machining technology, decellularized using 1% sodium dodecyl sulfate, and then opened longitudinally to form tissue sheets.

Antibacterial activity of chitosan-based matrices on oral pathogens.

Chitosan is a well sought-after polysaccharide in biomedical applications due to its biocompatibility, biodegradability to non-toxic substances, and ease of fabrication into various configurations. However, alterations in the anti-bacterial properties of chitosan in various forms is not completely understood. The objective of this study was to evaluate the anti-bacterial properties of chitosan matrices in different configurations against two pathogens-Gram-positive Streptococcus mutans and Gram-negative Actinobacillus actinomycetemcomitans.