Minimally Invasive Hemilaminectomy in Baastrup Disease: A Case Report and Discussion of "Kissing Spine Disease".

Case: A patient presented with midline back pain radiating into the left lower extremity. Magnetic Resonance images revealed an abnormal midline dorsal structure described as a facet cyst. Rupture was attempted by spinal needle under fluoroscopic guidance.

Targeting the PREX2/RAC1/PI3Kβ Signaling Axis Confers Sensitivity to Clinically Relevant Therapeutic Approaches in Melanoma.

Metastatic melanoma remains a major clinical challenge. Large-scale genomic sequencing of melanoma has identified bona fide activating mutations in RAC1, which are associated with resistance to BRAF-targeting therapies. Targeting the RAC1-GTPase pathway, including the upstream activator PREX2 and the downstream effector PI3Kβ, could be a potential strategy for overcoming therapeutic resistance, limiting melanoma recurrence, and suppressing metastatic progression.

Duality in disease: How two amino acid substitutions at actin residue 312 result in opposing forms of cardiomyopathy.

Two common types of cardiovascular disease are hypertrophic cardiomyopathy (HCM) and dilated cardiomyopathy (DCM) which occur from changes to sarcomere contractile mechanisms and activity. Actin amino acid substitutions R312C and R312H have been found in HCM and DCM patients, respectively. Previously, we observed that R312C/H variants display both hyperactivity and hypoactivity in vitro, contradicting traditional characterizations of HCM- and DCM-causing variants.

Identification of LRP1+CD13+ human periosteal stem cells that require LRP1 for bone repair.

Human periosteal skeletal stem cells (P-SSCs) are critical for cortical bone maintenance and repair. However, their in vivo identity, molecular characteristics, and specific markers remain unknown. Here, single-cell sequencing revealed human periosteum contains SSC clusters expressing known SSC markers, podoplanin (PDPN) and PDGFRA.

An ILK/STAT3 pathway controls glioblastoma stem cell plasticity.

Glioblastoma (GBM) is driven by malignant neural stem-like cells that display extensive heterogeneity and phenotypic plasticity, which drive tumor progression and therapeutic resistance. Here, we show that the extracellular matrix-cell adhesion protein integrin-linked kinase (ILK) stimulates phenotypic plasticity and mesenchymal-like, invasive behavior in a murine GBM stem cell model. ILK is required for the interconversion of GBM stem cells between malignancy-associated glial-like states, and its loss produces cells that are unresponsive to multiple cell state transition cues.

A lymphocyte chemoaffinity axis for lung, non-intestinal mucosae and CNS.

Tissue-selective chemoattractants direct lymphocytes to epithelial surfaces to establish local immune environments, regulate immune responses to food antigens and commensal organisms, and protect from pathogens. Homeostatic chemoattractants for small intestines, colon and skin are known, but chemotropic mechanisms selective for respiratory tract and other non-intestinal mucosal tissues remain poorly understood. Here we leveraged diverse omics datasets to identify GPR25 as a lymphocyte receptor for CXCL17, a chemoattractant cytokine whose expression by epithelial cells of airways, upper gastrointestinal and squamous mucosae unifies the non-intestinal mucosal tissues and distinguishes them from intestinal mucosae.

Anterioposterior Views Coupled With Lateral Views Are the Best for the Intraoperative Radiographic Detection of Retained Surgical Sponges.

Introduction: A retained sponge after spine surgery can cause serious medical complications and medicolegal problems. Intraoperative radiographs are commonly used to detect it. This study evaluated intraoperative radiographs under routine clinical conditions that most spine surgeons experience to detect retained sponges.

Mechanical characterization of Xenopus laevis oocytes using atomic force microscopy.

Mechanical properties are essential for the biological activities of cells, and they have been shown to be affected by diseases. Therefore, accurate mechanical characterization is important for studying the cell lifecycle, cell-cell interactions, and disease diagnosis. While the cytoskeleton and actin cortex are typically the primary structural stiffness contributors in most live cells, oocytes possess an additional extracellular layer known as the vitelline membrane (VM), or envelope, which can significantly impact their overall mechanical properties.

Removal of Spinal Instrumentation Is Not Required to Successfully Treat Postoperative Wound Infections in Most Cases.

Introduction: Controversy exists regarding whether spinal implants need to be removed to treat postoperative deep wound infections (DWIs). This retrospective study aimed to determine whether the removal or retention of implants impacts the successful treatment of a DWI after spine surgery.

Methods: Postoperative spine surgery patients presenting with signs of infection who underwent irrigation and debridement (I&D) at Twin Cities Spine Surgeons at Abbott Northwestern Hospital, Minnesota, USA, were studied.

Securing cloud data using secret key 4 optimization algorithm (SK4OA) with a non-linearity run time trend.

Cloud computing alludes to the on-demand availability of personal computer framework resources, primarily information storage and processing power, without the customer's direct personal involvement. Cloud computing has developed dramatically among many organizations due to its benefits such as cost savings, resource pooling, broad network access, and ease of management; nonetheless, security has been a major concern. Researchers have proposed several cryptographic methods to offer cloud data security; however, their execution times are linear and longer.

The application of the "inverse problem" method for constructing confining potentials that make N-soliton waveforms exact solutions in the Gross-Pitaevskii equation.

In this work, we discuss an application of the "inverse problem" method to find the external trapping potential, which has particular N trapped soliton-like solutions of the Gross-Pitaevskii equation (GPE) also known as the cubic nonlinear Schrödinger equation (NLSE). This inverse method assumes particular forms for the trapped soliton wave function, which then determines the (unique) external (confining) potential. The latter renders these assumed waveforms exact solutions of the GPE (NLSE) for both attractive (g<0) and repulsive (g>0) self-interactions.

High throughput screening identifies dasatinib as synergistic with trametinib in low grade serous ovarian carcinoma.

Background: Low grade serous ovarian carcinoma (LGSOC) is a distinct histotype of ovarian cancer characterised high levels of intrinsic chemoresistance, highlighting the urgent need for new treatments. High throughput screening in clinically-informative cell-based models represents an attractive strategy for identifying candidate treatment options for prioritisation in clinical studies.

Methods: We performed a high throughput drug screen of 1610 agents across a panel of 6 LGSOC cell lines (3 RAS/RAF-mutant, 3 RAS/RAF-wildtype) to identify novel candidate therapeutic approaches.

Morphological profiling in human neural progenitor cells classifies hits in a pilot drug screen for Alzheimer's disease.

Alzheimer's disease accounts for 60-70% of dementia cases. Current treatments are inadequate and there is a need to develop new approaches to drug discovery. Recently, in cancer, morphological profiling has been used in combination with high-throughput screening of small-molecule libraries in human cells .

Anti-obesity and metabolic benefits of metformin: Comparison of different delivery routes.

Obesity is a severe public health problem. Healthy lifestyle interventions are commonly recommended for fighting obesity. But they are hard to follow and have low efficacy.

Molecular determinants of antagonist interactions with chemokine receptors CCR2 and CCR5.

By driving monocyte chemotaxis, the chemokine receptor CCR2 shapes inflammatory responses and the formation of tumor microenvironments. This makes it a promising target in inflammation and immuno-oncology; however, despite extensive efforts, there are no FDA-approved CCR2-targeting therapeutics. Cited challenges include the redundancy of the chemokine system, suboptimal properties of compound candidates, and species differences that confound the translation of results from animals to humans.

Moderate scoliosis continues to progress at 30-year follow-up: a call for concern?

Purpose: This natural history study reports long-term radiographic and clinical outcomes of patients with diagnosis of AIS with curves between 30° and 50°. Our purpose was to determine if any intervention in the natural history is warranted.

Methods: This was a longitudinal descriptive study at a single institution.

Ensuring privacy and confidentiality of cloud data: A comparative analysis of diverse cryptographic solutions based on run time trend.

The cloud is becoming a hub for sensitive data as technology develops, making it increasingly vulnerable, especially as more people get access. Data should be protected and secured since a larger number of individuals utilize the cloud for a variety of purposes. Confidentiality and privacy of data is attained through the use of cryptographic techniques.

A multiscale predictive digital twin for neurocardiac modulation.

Cardiac function is tightly regulated by the autonomic nervous system (ANS). Activation of the sympathetic nervous system increases cardiac output by increasing heart rate and stroke volume, while parasympathetic nerve stimulation instantly slows heart rate. Importantly, imbalance in autonomic control of the heart has been implicated in the development of arrhythmias and heart failure.

Chemogenomic library design strategies for precision oncology, applied to phenotypic profiling of glioblastoma patient cells.

Designing a targeted screening library of bioactive small molecules is a challenging task since most compounds modulate their effects through multiple protein targets with varying degrees of potency and selectivity. We implemented analytic procedures for designing anticancer compound libraries adjusted for library size, cellular activity, chemical diversity and availability, and target selectivity. The resulting compound collections cover a wide range of protein targets and biological pathways implicated in various cancers, making them widely applicable to precision oncology.

Uniform-density Bose-Einstein condensates of the Gross-Pitaevskii equation found by solving the inverse problem for the confining potential.

In this work, we study the existence and stability of constant density (flat-top) solutions to the Gross-Pitaevskii equation (GPE) in confining potentials. These are constructed by using the "inverse problem" approach which corresponds to the identification of confining potentials that make flat-top waveforms exact solutions to the GPE. In the one-dimensional case, the exact solution is the sum of stationary kink and antikink solutions, and in the overlapping region, the density is constant.

Discovery of senolytics using machine learning.

Cellular senescence is a stress response involved in ageing and diverse disease processes including cancer, type-2 diabetes, osteoarthritis and viral infection. Despite growing interest in targeted elimination of senescent cells, only few senolytics are known due to the lack of well-characterised molecular targets. Here, we report the discovery of three senolytics using cost-effective machine learning algorithms trained solely on published data.