Publications by authors named "John C Charpentier"
Once thought of primarily as a means to neutralize pathogens or to facilitate feeding, endocytosis is now known to regulate a wide range of eukaryotic cell processes. Among these are regulation of signal transduction, mitosis, lipid homeostasis, and directed migration, among others. Less well-appreciated are the roles various forms of endocytosis plays in regulating αβ and, especially, γδ T cell functions, such as T cell receptor signaling, antigen discovery by trogocytosis, and activated cell growth.
View Article and Find Full Text PDFVav family guanine nucleotide exchange factors (GEFs) are essential regulators of immune function. Despite their structural similarity, Vav1 promotes and Vav2 opposes T cell receptor (TCR)-induced Ca entry. By using a Vav1-deficient Jurkat T cell line, we find that Vav1 facilitates Ca entry via non-catalytic scaffolding functions that are encoded by the catalytic core of Vav1 and flanking linker regions.
View Article and Find Full Text PDFArticle Synopsis
- Macropinocytosis is a form of endocytosis that allows large-scale uptake of fluids and nutrients, playing roles in various biological processes like immune responses and tumor growth regulation.
- Researchers found that T cells also use macropinocytosis, especially when activated, to enhance their growth, even when amino acids are plentiful.
- The process helps transport extracellular amino acids into a specific cell compartment, aiding in the activation of the mTORC1 pathway, which is crucial for T cell growth and indicates that macropinocytosis has broader implications for non-cancerous cell growth.