Publications by authors named "John Bonkowski"

Erwinia pyrifoliae causes disease of pear (Pyrus spp.), apple (Malus spp.), and strawberry (Fragaria × ananassa) (Wenneker and Bergsma-Vlami 2015), which are economically important commodities in the US.

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Eastern redbud ( L.) is a popular and high-value woody ornamental plant native to the eastern and south-central United States of America (U.S.

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Article Synopsis
  • Soybean samples from Indiana showed signs of severe disease, including leaf chlorosis, dark lesions on stems, and root rot, affecting over 50% of plants and resulting in significant yield loss.
  • Fungal analysis revealed growth of specific fungal colonies with distinct characteristics from the infected stem tissues, indicating a possible pathogen.
  • DNA extraction and gene amplification were performed on the isolated fungus, confirming its identity through sequencing, marking it as a potential threat to soybean crops in the region.*
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Tar spot, a disease caused by the ascomycete fungal pathogen , is considered one of the most significant yield-limiting diseases of maize () within the United States. may also be found in association with other fungi, forming a disease complex that is thought to result in the characteristic fisheye lesions. Understanding how colonizes maize leaf cells is essential for developing effective disease control strategies.

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The soybean cyst nematode (SCN, ) is the most yield-limiting pathogen of soybean in the US. This study was carried out in order to provide updated information on SCN virulence phenotypes in Indiana. A total of 124 soil samples were collected from soybean fields in 2020 and all of them tested positive for SCN.

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Globalization has made agricultural commodities more accessible, available, and affordable. However, their global movement increases the potential for invasion by pathogens and necessitates development and implementation of sensitive, rapid, and scalable surveillance methods. Here, we used 35 strains, isolated by multiple diagnostic laboratories, as a case study for using whole genome sequence data in a plant disease diagnostic setting.

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Soybeans () are an important crop for Indiana, playing a major role in the state's economy. In June 2021, symptomatic soybean plants were submitted to Purdue University's Plant and Pest Diagnostic Laboratory for diagnosis. Sting nematodes were observed on the surface of the washed roots using stereo- and brightfield compound microscopy.

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Plant diagnostic laboratories (PDLs) are at the heart of land-grant universities (LGUs) and their extension mission to connect citizens with research-based information. Although research and technological advances have led to many modern methods and technologies in plant pathology diagnostics, the pace of adopting those methods into services at PDLs has many complexities we aim to explore in this review. We seek to identify current challenges in plant disease diagnostics, as well as diagnosticians' and administrators'perceptions of PDLs' many roles.

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In 2012, stem gall samples on Loropetalum chinense were sent to Florida diagnostic labs from Alabama and Florida nurseries. A fluorescent pseudomonad was consistently isolated from the galls. The organism was originally identified in Alabama based on 16S rRNA sequencing as Pseudomonas savastanoi, which causes a production-limiting disease of olive.

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