Publications by authors named "John Austad"

Article Synopsis
  • A study published in J. AOAC Int. detailed a method for measuring total myo-inositol in infant and nutritional formulas using high-performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD).
  • The AOAC Expert Review Panel approved this method as Official First Action, confirming it meets the required standards for free myo-inositol determination while also including total myo-inositol analysis.
  • The method features two techniques: a traditional approach involving 6 hours in an autoclave and a more efficient microwave-assisted acid hydrolysis with enzymatic treatment that shortens extraction time.
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A method for the analysis of free and total myo-inositol in foods, feeds, and infant formulas has been developed and validated using high-performance anion exchange chromatography with pulsed amperometric detection. The option of a free myo-inositol determination or a complete total myo-inositol determination from main bound sources can be achieved. These sources include phytates, lower'phosphorylated forms, and phosphatidylinositol.

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A collaborative study was conducted to evaluate the interlaboratory performance of an LC method for lycopene in dietary supplements and the raw materials commonly used in their manufacture. Twelve laboratories from six countries agreed to participate in the study. Results from 10 laboratories were received and are reported.

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The presence of aristolochic acid in some dietary supplements is a concern to regulators and consumers. A method has been developed, by initially using a reference method as a guide, during single laboratory validation (SLV) for the determination of aristolochic acid I, also known as aristolochic acid A, in botanical species and dietary supplements at concentrations of approximately 2 to 32 microg/g. Higher levels were determined by dilution to fit the standard curve.

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