Description of Cohnella rhizoplanae sp. nov., isolated from the root surface of soybean (Glycine max).

A Gram-staining-positive, aerobic bacterium, designated strain JJ-181, was isolated from the root surface of soybean. Based on the 16S rRNA gene sequence similarities, strain JJ-181 was grouped into the genus Cohnella, most closely related to Cohnella hashimotonis F6_2S_P_1 (98.85%) and C.

sp. nov. and sp. nov., isolated from the rhizosphere of .

Two Gram-stain-positive, aerobic, endospore-forming bacterial strains, isolated from the rhizosphere of were studied for their detailed taxonomic allocation. Based on 16S rRNA gene sequence similarity comparisons, both strains JJ-7 and JJ-60 were shown to be members of the genus . Strain JJ-7 was most closely related to the type strains of (99.

sp. nov., isolated from the rhizosphere, and reclassification of as comb. nov.

A Gram-stain-positive, facultative anaerobic endospore-forming bacterium, which originated from roots/rhizosphere of maize (), was investigated for its taxonomic position. On the basis of 16S rRNA gene sequence similarities, strain JJ-3 was grouped together with species showing the highest similarities to (98.8 %) and the three species , and (all 98.

sp. nov. isolated from roots.

An aerobic, Gram-staining-positive, endospore-forming bacterium, isolated from the rhizosphere of roots of maize (), was taxonomically studied. On the basis of 16S rRNA gene sequence similarity comparisons, strain JJ-125 clustered together with species of the genus and showed the highest similarities with (98.7 %).

Paenibacillus allorhizoplanae sp. nov. from the rhizoplane of a Zea mays root.

A Gram-positive staining, aerobic, endospore-forming bacterial strain, isolated from the rhizosphere of Zea mays was studied for its detailed taxonomic allocation. Based on the 16S rRNA gene sequence similarity comparisons, strain JJ-42 was shown to be a member of the genus Paenibacillus, most closely related to the type strain of Paenibacillus pectinilyticus (98.8%).

sp. nov. from maize roots.

A Gram-stain-positive, aerobic and endospore-forming bacterial strain, isolated from the root surface of maize () was taxonomically studied. It could be clearly shown that, based on 16S rRNA gene sequence similarity comparisons, strain JJ-63 is a member of the genus , most closely related to the type strain of (98.61%), followed by (98.

gen. nov., sp. nov., isolated from maize root rhizosphere.

A facultative anaerobic, Gram-stain-positive, endospore-forming bacterium, isolated from the rhizosphere of maize roots (), was taxonomically studied. Based on 16S rRNA gene sequence similarity comparisons, strain JJ-79 clustered only loosely with species and showed the highest similarity to (97.9%).

sp. nov., isolated from charcoal.

A beige-pigmented, oxidase-positive bacterial isolate, Wesi-4, isolated from charcoal in 2012, was examined in detail by applying a polyphasic taxonomic approach. Cells of the isolates were rod shaped and Gram-stain negative. Examination of the 16S rRNA gene sequence of the isolate revealed highest sequence similarities to the type strains of and (both 97.

sp. nov. isolated from the rhizosphere of the switchgrass .

A Gram-staining-negative non endospore-forming strain, PXU-55, was isolated from the rhizosphere of the switchgrass and studied in detail to determine its taxonomic position. The results of 16S rRNA gene sequence analysis indicated that the isolate represented a member of the genus . The isolate shared highest 16S rRNA gene sequence similarities with the type strains of (98.

Xinfangfangia humi sp. nov., isolated from soil amended with humic acid.

Two slightly beige-pigmented, Gram-stain-negative, rod-shaped bacterial strains, IMT-291 and IMT-297, were isolated from soil in a field located in Malvern, Alabama, USA. The source soil had been amended with humic acid and continuously used for the cultivation of worms used for fish bait. It is still conceivable that the source of the strains is from the humic acid amendment, although all attempts to isolate the novel phenotypes from the humic acid source have failed.

Pectin-Rich Amendment Enhances Soybean Growth Promotion and Nodulation Mediated by Strains.

Plant growth-promoting rhizobacteria (PGPR) are increasingly used in crops worldwide. While selected PGPR strains can reproducibly promote plant growth under controlled greenhouse conditions, their efficacy in the field is often more variable. Our overall aim was to determine if pectin or orange peel (OP) amendments to (Bv) PGPR strains could increase soybean growth and nodulation by in greenhouse and field experiments to reduce variability.

Pigmentiphaga humi sp. nov., isolated from soil amended with humic acid.

A slightly beige-pigmented, Gram-stain-negative, rod-shaped bacterium, strain IMT-318, was isolated from soil in a field located in Malvern, Alabama, USA. Phylogenetic analysis based on the 16S rRNA gene placed the strain within the genus Pigmentiphaga with highest 16S rRNA gene sequence similarity of 98.74 % and 98.

Filibacter tadaridae sp. nov., isolated from within a guano pile from a colony of Mexican free-tailed bats Tadarida brasiliensis.

A Gram-stain-positive, aerobic bacterium, TB-66, was isolated from a pile of bat guano in a cave of New Mexico, USA. On the basis of 16S rRNA gene sequence similarity comparisons, strain TB-66grouped together with Filibacter limicola showing a 16S rRNA gene sequence similarity of 98.5 % to the type strain.

Mixtures of Plant-Growth-Promoting Rhizobacteria Enhance Biological Control of Multiple Plant Diseases and Plant-Growth Promotion in the Presence of Pathogens.

Several studies have shown that mixtures of plant-growth-promoting rhizobacteria (PGPR) could enhance biological control activity for multiple plant diseases through the mechanisms of induced systemic resistance or antagonism. Prior experiments showed that four individual PGPR strains-AP69 (Bacillus altitudinis), AP197 (B. velezensis), AP199 (B.

Variation in Bat Guano Bacterial Community Composition With Depth.

Bats are known to be reservoirs for a variety of mammalian pathogens, including viruses, fungi, and bacteria. Many of the studies examining the microbial community inhabiting bats have investigated bacterial taxa found within specific bat tissues and isolated bat guano pellets, but relatively few studies have explored bacterial diversity within bat guano piles. In large bat caves, bat guano can accumulate over time, creating piles several meters deep and forming complex interactions with coprophagous organisms in a habitat with low light and oxygen.

Isolation and characterization of N -fixing bacteria from giant reed and switchgrass for plant growth promotion and nutrient uptake.

The aims of this study were to isolate and characterize N -fixing bacteria from giant reed and switchgrass and evaluate their plant growth promotion and nutrient uptake potential for use as biofertilizers. A total of 190 bacteria were obtained from rhizosphere soil and inside stems and roots of giant reed and switchgrass. All the isolates were confirmed to have nitrogenase activity, 96.

Paenibacillus nebraskensis sp. nov., isolated from the root surface of field-grown maize.

A Gram-positive-staining, aerobic, non-endospore-forming bacterial strain (JJ-59), isolated from a field-grown maize plant in Dunbar, Nebraska in 2014 was studied by a polyphasic approach. Based on 16S rRNA gene sequence similarity comparisons, strain JJ-59 was shown to be a member of the genus Paenibacillus, most closely related to the type strains of Paenibacillus aceris (98.6 % 16S rRNA gene sequence similarity) and Paenibacillus chondroitinus (97.

Flavobacterium gossypii sp. nov. isolated from the root tissue of field-grown cotton.

A yellow-pigmented bacterial strain, JM-222T, isolated from the root tissue of field-grown cotton (Gossypium hirsutum) in Auburn, Alabama, USA, was studied for its taxonomic allocation. Cells of the isolate were rod-shaped and Gram-stain-negative. A blast analysis using the EzTaxon database showed that strain JM-222T shared highest 16S rRNA gene sequence similarity with the type strain of Flavobacterium endophyticum (98.

Biological control of Heterodera glycines by spore-forming plant growth-promoting rhizobacteria (PGPR) on soybean.

Heterodera glycines, the soybean cyst nematode, is the most economically important plant-parasitic nematode on soybean production in the U.S. The objectives of this study were to evaluate the potential of plant growth-promoting rhizobacteria (PGPR) strains for mortality of H.

Paracandidimonas soli gen. nov., sp. nov., isolated from soil.

A slightly yellow-pigmented, Gram-stain-negative, rod-shaped bacterium, strain IMT-305T, was isolated from soil in Alabama, USA. Phylogenetic analysis based on the nearly full-length 16S rRNA gene sequence placed the strain in between the genera Pusillimonas, Parapusillimonas and Candidimonas with highest 16S rRNA gene sequence similarity to the type strain of Parapusillimonas granuli (97.5 %) and Candidimonas nitroreducens (97.

Biological Control of Meloidogyne incognita by Spore-forming Plant Growth-promoting Rhizobacteria on Cotton.

In the past decade, increased attention has been placed on biological control of plant-parasitic nematodes using various fungi and bacteria. The objectives of this study were to evaluate the potential of 662 plant growth-promoting rhizobacteria (PGPR) strains for mortality to Meloidogyne incognita J2 in vitro and for nematode management in greenhouse, microplot, and field trials. Results indicated that the mortality of M.

Selection and Assessment of Plant Growth-Promoting Rhizobacteria for Biological Control of Multiple Plant Diseases.

A study was designed to screen individual strains of plant growth-promoting rhizobacteria (PGPR) for broad-spectrum disease suppression in vitro and in planta. In a preliminary screen, 28 of 196 strains inhibited eight different tested pathogens in vitro. In a secondary screen, these 28 strains showed broad spectrum antagonistic activity to six different genera of pathogens, and 24 of the 28 strains produced five traits reported to be related to plant growth promotion, including nitrogen fixation, phosphate solubilization, indole-3-acetic acid production, siderophore production, and biofilm formation.