Publications by authors named "Johari W"

Unlabelled: A consortium of bacteria capable of decomposing oily hydrocarbons was isolated from tarballs on the beaches of Terengganu, Malaysia, and classified as , , and . The Taguchi design was used to optimize the biodegradation of diesel using these bacteria as a consortium. The highest biodegradation of diesel-oil in the experimental tests was 93.

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Propolis is a resinous substance collected by stingless bees containing bioactive compounds which exert various biological properties. The present study focused on the evaluation of chemical profiles produced by three Indo-Malayan stingless bee propolis extracted using water. Fresh propolis was collected from the same area and ecosystem conditions in Selangor, Malaysia, namely , , and .

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Article Synopsis
  • Researchers discovered two cold-resistant bacteria from pristine Antarctic soil, capable of using diesel fuel for energy.
  • Findings indicated that both strains, closely related to Pseudomonas and Rhodococcus, thrived at an optimal temperature of 20°C and a pH of 7, with strain ADL36 exhibiting superior diesel degradation capabilities.
  • Under optimized conditions, strain ADL36 achieved nearly complete mineralization of n-dodecane, outperforming strain ADL15 significantly.
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In this study, we isolated two indigenous hydrocarbon-degrading bacteria from tarball found in Rhu Sepuluh beach, Terengganu, Malaysia. These bacteria were identified based on their physiological characteristic and 16S rRNA gene sequence analysis, and they showed 99% similarity with Cellulosimicrobium cellulans DSM 43879 and Acinetobacter baumannii ATCC 19606 respectively. Their hydrocarbon-degrading capabilities were tested using diesel-oil as sole carbon source.

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In a previous study, we isolated Leifsonia sp. strain SIU, a new bacterium from agricultured soil. The bacterium was tested for its ability to degrade caffeine.

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A possible tributyltin (TBT)-degrading bacterium isolated from contaminated surface sediment was successfully identified as Klebsiella sp. FIRD 2. It was found to be the best isolate capable of resisting TBT at a concentration of 1000 μg L(-1).

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Article Synopsis
  • Luminescence-based assays for detecting toxicants like Microtox and ToxAlert have limitations in tropical climates due to temperature issues.
  • A new isolate from mackerel, identified as Photobacterium sp. strain MIE, shows high luminescence activity in a suitable temperature range of 24-30°C and specific nutrient conditions.
  • The Xenoassay light system using this strain demonstrated the ability to monitor heavy metals in real-time, yielding results comparable to standard assays in under 30 minutes, making it effective for river monitoring in tropical areas.
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A diesel-degrading bacterium was isolated from a diesel-contaminated site in Selangor, Malaysia. The isolate was tentatively identified as Acinetobacter sp. strain DRY12 based on partial 16S rDNA molecular phylogeny and Biolog GN microplate panels and Microlog database.

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Molybdenum is very toxic to agricultural animals. Mo-reducing bacterium can be used to immobilize soluble molybdenum to insoluble forms, reducing its toxicity in the process. In this work the isolation of a novel molybdate-reducing Gram positive bacterium tentatively identified as Bacillus sp.

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A one-dimensional model has been evaluated for transport of hydrophobic contaminants, such as polycyclic aromatic hydrocarbon (PAH) compounds, facilitated by synthetic amphiphilic polyurethane (APU) nanoparticles in porous media. APU particles synthesized from poly(ethylene glycol)-modified urethane acrylate (PMUA) precursor chains have been shown to enhance the desorption rate and mobility of phenanthrene (PHEN) in soil. A reversible process governed by attachment and detachment rates was considered to describe the PMUA binding in soil in addition to PMUA transport through advection and dispersion.

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