Virulence Profiles and Genome-Wide Association Study for Isolates Collected from Australian Lentil-Growing Regions.

, the causal organism of Ascochyta blight (AB) of lentil (), has been shown to produce an avirulence effector protein that mediates AB resistance in certain lentil cultivars. The two known forms of the effector protein were identified from a biparental mapping population between isolates that have reciprocal virulence on 'PBA Hurricane XT' and 'Nipper'. The effector AlAvr1-1 was described for the PBA Hurricane XT-avirulent isolate P94-24 and AlAvr1-2 characterized in the PBA Hurricane XT-virulent isolate Kewell.

Phenotypic and Genotypic Diversity of Populations in Southern Australia.

Speg. is a serious foliar fungal disease of faba bean and a constraint to production worldwide. This study investigated the phenotypic and genotypic diversity of the pathogen population in southern Australia and the pathogenic variability of the population was examined on a differential set of faba bean cultivars.

The novel avirulence effector AlAvr1 from Ascochyta lentis mediates host cultivar specificity of ascochyta blight in lentil.

Ascochyta lentis is a fungal pathogen that causes ascochyta blight in the important grain legume species lentil, but little is known about the molecular mechanism of disease or host specificity. We employed a map-based cloning approach using a biparental A. lentis population to clone the gene AlAvr1-1 that encodes avirulence towards the lentil cultivar PBA Hurricane XT.

An automated and combinative method for the predictive ranking of candidate effector proteins of fungal plant pathogens.

Fungal plant-pathogens promote infection of their hosts through the release of 'effectors'-a broad class of cytotoxic or virulence-promoting molecules. Effectors may be recognised by resistance or sensitivity receptors in the host, which can determine disease outcomes. Accurate prediction of effectors remains a major challenge in plant pathology, but if achieved will facilitate rapid improvements to host disease resistance.

Agroinfiltration for transient gene expression and characterisation of fungal pathogen effectors in cool-season grain legume hosts.

Modified pEAQ-HT-DEST1 vectors were used for agroinfiltration in legumes. We demonstrate protein expression and export in pea, lentil, and faba bean; however, the method for chickpea was not successful. Agroinfiltration is a valuable research method for investigating virulence and avirulence effector proteins from pathogens and pests, where heterologous effector proteins are transiently expressed in plant leaves and hypersensitive necrosis responses and other effector functions can be assessed.

Reference genome assembly for Australian Ascochyta lentis isolate Al4.

Ascochyta lentis causes ascochyta blight in lentil (Lens culinaris Medik.) and yield loss can be as high as 50%. With careful agronomic management practices, fungicide use, and advances in breeding resistant lentil varieties, disease severity and impact to farmers have been largely controlled.

Targeted Disruption of Scytalone Dehydratase Gene Using -Mediated Transformation Leads to Altered Melanin Production in .

Sustainable crop production is constantly challenged by the rapid evolution of fungal pathogens equipped with an array of host infection strategies and survival mechanisms. One of the devastating fungal pathogens that infect lentil is the ascomycete which causes black spot or ascochyta blight (AB) on all above ground parts of the plant. In order to explore the mechanisms involved in the pathogenicity of , we developed a targeted gene replacement method using mediated transformation (ATMT) to study and characterize gene function.

Reference Genome Assembly for Australian Isolate ArME14.

is the causal organism of ascochyta blight of chickpea and is present in chickpea crops worldwide. Here we report the release of a high-quality PacBio genome assembly for the Australian isolate ArME14. We compare the ArME14 genome assembly with an Illumina assembly for Indian isolate, ArD2.

Characterization of Growth Morphology and Pathology, and Draft Genome Sequencing of , the Causal Organism of Chocolate Spot of Faba Bean ( L.).

Chocolate spot is a major fungal disease of faba bean caused by the ascomycete fungus, . is also implicated in botrytis gray mold disease in lentils, along with . Here we have isolated and characterized two isolates from chocolate spot lesions on faba bean leaves.

Agrobacterium tumefaciens-mediated transformation and expression of GFP in Ascochyta lentis to characterize ascochyta blight disease progression in lentil.

The plant immune system is made up of a complex response network that involves several lines of defense to fight invading pathogens. Fungal plant pathogens on the other hand, have evolved a range of ways to infect their host. The interaction between Ascochyta lentis and two lentil genotypes was explored to investigate the progression of ascochyta blight (AB) in lentils.

Activation of the two microRNA clusters C19MC and miR-371-3 does not play prominent role in thyroid cancer.

Chromosomal rearrangements of band 19q13.4 are frequent cytogenetic alterations in benign thyroid adenomas. Apparently, these alterations lead to the upregulation of genes encoding microRNAs of two clusters mapping to the breakpoint region, i.