Shaping Liposomes by Cell-Free Expressed Bacterial Microtubules.

Genetic control over a cytoskeletal network inside lipid vesicles offers a potential route to controlled shape changes and DNA segregation in synthetic cell biology. Bacterial microtubules (bMTs) are protein filaments found in bacteria of the genus . They are formed by the tubulins BtubA and BtubB, which polymerize in the presence of GTP.

Modelling cell-free RNA and protein synthesis with minimal systems.

DNA-guided cell-free protein synthesis using a minimal set of purified components has emerged as a versatile platform in constructive biology. The E. coli-based PURE (protein synthesis using recombinant elements) system offers the basic protein synthesis factory in a prospective minimal cell relying on extant molecules.