Multispectral tracing in densely labeled mouse brain with nTracer.

Summary: This note describes nTracer, an ImageJ plug-in for user-guided, semi-automated tracing of multispectral fluorescent tissue samples. This approach allows for rapid and accurate reconstruction of whole cell morphology of large neuronal populations in densely labeled brains.

Availability And Implementation: nTracer was written as a plug-in for the open source image processing software ImageJ.