Publications by authors named "Johanna Raffel"
Article Synopsis
- - SARS-CoV-2 causes COVID-19, and while both genomic RNA (gRNA) and subgenomic RNAs (sgRNAs) are produced in infected cells, their roles in measuring active virus replication and predicting infectivity are debated.
- - Current methods to monitor SARS-CoV-2 infections primarily use RT-qPCR to detect gRNA, but the relationship between viral load and infectivity depends on the assay's performance, as Ct-values may not accurately reflect the presence of active viruses.
- - A study developed a multiplex RT-qPCR assay detecting both gRNA and sgRNA, finding no significant predictive advantage in using sgRNAs; in fact, gRNA alone was slightly more reliable for determining viral infectivity,
The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the causative agent of the acute respiratory disease COVID-19, which has become a global concern due to its rapid spread. The common methods to monitor and quantitate SARS-CoV-2 infectivity in cell culture are so far time-consuming and labor-intensive. Using the Sleeping Beauty transposase system, we generated a robust and versatile cellular infection model that allows SARS-CoV-2 infection experiments compatible for high-throughput and live cell imaging.
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