Publications by authors named "Johanna Koskivuori"
Objectives: Hospital pharmacies provide centralised intravenous additive services (CIVAS), such as antibiotic reconstitution. The aim of this study was to demonstrate the physicochemical stability of high-concentration cefuroxime sodium in aqueous injections, which is mandatory for the centralised preparation of products with automation.
Methods: The physicochemical stability of three high-concentration injections (1.
Previous steroid hormone studies concerning pregnancy and newborns have mainly focused on glucocorticoids; wider steroid profiles have been less commonly investigated. Here, we performed a comparative analysis of 17 steroids from newborn hair and umbilical cord serum at the time of delivery. The study participants (n = 42, 50% girls) were a part of the Kuopio Birth Cohort and represent usual Finnish pregnancies.
View Article and Find Full Text PDFArticle Synopsis
- The study investigates how steroid concentrations in endometrial tissue and serum relate to gene expression of steroid-metabolizing enzymes to assess endometrial receptivity in IVF patients.
- It involves a case-control design with 40 IVF patients, comparing 20 women who achieved clinical pregnancy to 20 who did not, while controlling for various factors like fertility type and age.
- Results show no overall differences in steroid levels between pregnant and nonpregnant groups, but pregnant women with primary infertility had lower estrone levels and a distinct estrone:androstenedione ratio compared to their nonpregnant counterparts.
Hair steroid analysis offers the possibility to evaluate long-term steroid metabolism. It is especially beneficial when studying the steroid milieu in newborns and children, for example it can provide new insights into steroid metabolism over months and is unaffected by diurnal fluctuations in steroid concentrations. This study describes a quantitative and highly selective high-resolution mass spectrometry method for the simultaneous analysis of multiple steroids from human scalp hair.
View Article and Find Full Text PDFObjective: To characterize serum biomarkers in mitochondrial CHCHD10-linked spinal muscular atrophy Jokela (SMAJ) type for disease monitoring and for the understanding of pathogenic mechanisms.
Methods: We collected serum samples from a cohort of 49 patients with SMAJ, all carriers of the heterozygous c.197G>T p.
This study describes a validated LC-MS/MS method for assaying 23 steroids within a single run from 150 μl of human plasma, serum or prostatic tissue homogenate. Isotope-labeled steroids were used as internal standards. Samples were extracted with toluene, and ketosteroids were derivatized with hydroxylamine prior to LC-MS/MS analysis.
View Article and Find Full Text PDF