Immunohistochemical detection of interleukin-8 in inflamed porcine tissues.

The objective of this study was to identify the specific localization of interleukin-8 (IL-8) in cells in situ in a variety of inflammatory processes in different tissues from pigs. Our hypothesis was that IL-8 primarily is a neutrophil related cytokine present in all extravascular neutrophils while expression also occurs in other cell types in response to an inflammatory stimulus. Using IL-8 immunohistochemistry we discovered that neutrophils were the predominant IL-8 positive cell population while epithelial cell types and endothelium of postcapillary venules could be positive when situated in close vicinity of an inflammatory lesion.

Porcine models of non-bacterial thrombotic endocarditis (NBTE) and infective endocarditis (IE) caused by Staphylococcus aureus: a preliminary study.

Background And Aim Of The Study: Non-bacterial thrombotic endocarditis (NBTE) and, in particular, infective endocarditis (IE), are serious and potentially life-threatening diseases. An increasingly important agent of human IE is Staphylococcus aureus, which typically causes an acute endocarditis with high mortality. The study aim was to evaluate the pig as a model for non-bacterial as well as S.

Embolic encephalitis in a porcine model of endocarditis.

Background: Endocarditis is a severe disease in which neurological complications are frequent and associated with increased mortality and complex disease management. In the present study, the pig was evaluated as a model of embolic encephalitis as a complication of experimental infective endocarditis.

Materials And Methods: Brains from pigs with experimental Staphylococcus aureus-associated infective endocarditis (IE; n=2), experimental non-bacterial thrombotic endocarditis (NBTE; n=5), experimental S.

Systemic inflammatory response and local cytokine expression in porcine models of endocarditis.

The knowledge of systemic inflammation and local cytokine expression in porcine endocarditis models is limited, though it could provide valuable information about the pathogenesis and comparability to human endocarditis. Analyses of bacteriology and hematology were performed on blood samples from pigs with non-bacterial thrombotic endocarditis (NBTE, n = 11), Staphylococcus aureus infective endocarditis (IE, n = 2), animals with S. aureus sepsis without endocarditis (n = 2) and controls (n = 2).