The enteron Escherichia coli is equipped with a branched electron transfer chain that mediates chemiosmotic electron transfer, that drives ATP synthesis. The components of this electron transfer chain couple the oxidation of available electron donors from cellular metabolism (e.g.
View Article and Find Full Text PDFUnlabelled: Intracellular pH (pH) critically affects bacterial cell physiology. Hence, a variety of food preservation strategies are aimed at perturbing pH homeostasis. Unfortunately, accurate pH quantification with existing methods is suboptimal, since measurements are averages across populations of cells, not taking into account interindividual heterogeneity.
View Article and Find Full Text PDFAims: Carbon monoxide is a respiratory poison and gaseous signaling molecule. Although CO-releasing molecules (CORMs) deliver CO with temporal and spatial specificity in mammals, and are proven antimicrobial agents, we do not understand the modes of CO toxicity. Our aim was to explore the impact of CO gas per se, without intervention of CORMs, on bacterial physiology and gene expression.
View Article and Find Full Text PDFThe internal pH (pHi) of a living cell is one of its most important physiological parameters. To monitor the pH inside Bacillus subtilis during various stages of its life cycle, we constructed an improved version (IpHluorin) of the ratiometric, pH-sensitive fluorescent protein pHluorin by extending it at the 5' end with the first 24 bp of comGA. The new version, which showed an approximate 40% increase in fluorescence intensity, was expressed from developmental phase-specific, native promoters of B.
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