Publications by authors named "Johan Harmse"

Article Synopsis
  • Researchers have uncovered significant molecular complexity in synapses, identifying over 1,000 proteins within the synapse proteome, but the diversity of synapses across the brain is still not well understood due to current research limitations.
  • This study introduces an efficient method for purifying synaptic protein complexes by tagging PSD95, allowing for visualization in specific cell types and showcasing differences in PSD95 complexes between specific neurons and diverse tissues.
  • The findings suggest that synaptic protein interactions vary by brain region and cell type, providing new tools for further investigation into synapse diversity and potential implications for understanding psychiatric and neurological diseases.
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Inverted repeat (IR) RNA silencing vectors containing homologous fragments of target endogenous plant genes, or pathogen genes, are the most widely used vectors to either study the function of genes involved in biotic stress or silence pathogens to induce plant resistance, respectively. RNA silencing has been exploited to produce transgenic plants with resistance to viral pathogens via posttranscriptional gene silencing (PTGS). In some cases, this technology is difficult to apply due to the instability of IR constructs during cloning and plant transformation.

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RNA silencing has been exploited to produce transgenic plants with resistance to viral pathogens via posttranscriptional gene silencing (PTGS). In some cases, this technology is difficult to apply due to the instability of inverted repeat (IR) constructs during cloning and plant transformation. Although such constructs have been shown to be stabilized with introns and efficiently induce RNA silencing, we found that the Pdk intron did not stabilize South African cassava mosaic virus (SACMV) silencing constructs.

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Integrative and conjugative elements (ICEs) are self-mobile genetic elements found in the genomes of some bacteria. These elements may confer a fitness advantage upon their host bacteria through the cargo genes that they carry. Salmonella pathogenicity island 7 (SPI-7), found within some pathogenic strains of Salmonella enterica, possesses features indicative of an ICE and carries genes implicated in virulence.

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