Room Temperature Intrinsic Emission Ratio of BSA Correlates With Percent Aggregates During Long-Term Storage.

Successful formulation development hinges on the ability to screen and identify excipients that stabilize drug products during long-term storage. Biophysical and accelerated stability studies are used to screen for excipients that stabilize protein drug products. However, these studies are not always predictive of aggregation during long-term storage.

Identification of Chemical Compounds That Inhibit the Function of Glutamyl-tRNA Synthetase from Pseudomonas aeruginosa.

Pseudomonas aeruginosa glutamyl-tRNA synthetase (GluRS) was overexpressed in Escherichia coli. Sequence analysis indicated that P. aeruginosa GluRS is a discriminating GluRS and, similar to other GluRS proteins, requires the presence of tRNA(Glu) to produce a glutamyl-AMP intermediate.

An apparatus to control and automate the formation of continuous density gradients.

A common problem associated with making a continuous density gradient is the unwanted mixing of liquids as the gradient solution is being delivered from the gradient mixer to the centrifuge tube. We show that by using a Styrofoam float on a thin drawn-out glass pipette, liquids from the gradient mixer will flow down the outside surface of the glass fiber and contact the float, after which the liquid spreads across the gradient surface without unwanted mixing. As the liquid in the tube rises, the float rises up the glass fiber, thereby allowing the creation of gradients without the need to monitor the process.