In vitro assessment of the genotoxicity and immunotoxicity of treated and untreated municipal effluents and receiving waters in freshwater organisms.

Municipal wastewater effluent is one of the largest sources of pollution entering surface waters in the Laurentian Great Lakes. Exposure to wastewater effluent has been associated with impaired immune systems and induction of genotoxicity to aquatic animals. Due to habitat degradation and environmental pollution linked to industrial development and population growth, several regions of the Great Lakes have been designated Areas of Concern (AOCs).

Toxicity and bioconcentration of the pharmaceuticals moxifloxacin, rosuvastatin, and drospirenone to the unionid mussel Lampsilis siliquoidea.

Pharmaceuticals and personal care products (PPCPs) and their metabolites are continually released from wastewater treatment plants into the aquatic environment; however, their impact on aquatic biota is poorly understood. This study examined the toxicity and bioconcentration of three pharmaceuticals: moxifloxacin, rosuvastatin, and drospirenone to the unionid mussel Lampsilis siliquoidea. Effects of moxifloxacin and rosuvastatin were assessed through aqueous 21-d static-renewal tests using 2-year-old mussels, at 0.

In situ analysis of cadmium uptake in four sections of the gastro-intestinal tract of rainbow trout (Oncorhynchus mykiss).

This study links results from past in vitro and in vivo experiments, by implementing an in situ experiment in order to determine the relative importance for cadmium (Cd) uptake of different sections of the gastro-intestinal tract (GIT) of rainbow trout. Transport of Cd from four sections of the GIT of adult rainbow trout (~220 g) was individually examined by infusing ligated sections of the GIT in live, free-swimming fish with 50 μM Cd spiked with radiolabelled (109)Cd (0.5 μCi ml(-1)).

Gastro-intestinal transport of calcium and cadmium in fresh water and seawater acclimated trout (Oncorhynchus mykiss).

Transport of calcium (Ca) and cadmium (Cd) was examined along the gastro-intestinal tract (GIT) of freshwater and seawater Oncorhynchus mykiss irideus (FWT and SWTies respectively) using in vitro and in vivo experiments. Based on known physiological differences between FWT and SWT which aid in regulating ion levels and osmolarity, we hypothesized that SWT would have lower rates of Ca uptake. Also, we predicted that Cd rates would also be lower because Cd is known to share a common transport mechanism with Ca.

In vitro characterization of cadmium transport along the gastro-intestinal tract of freshwater rainbow trout (Oncorhynchus mykiss).

An in vitro gut sac technique was used to examine the mechanism(s) of cadmium (Cd) uptake along the gastro-intestinal tract (GIT) of rainbow trout (Oncorhynchus mykiss). The spatial distribution of Cd between three compartments (mucus-binding, mucosal epithelium, and transport into blood space) was determined using a modified Cortland saline containing 50μM Cd (as CdCl(2)) labeled with (109)Cd radiotracer. Taking into account total surface areas, the order of relative importance for total Cd uptake rate was: posterior intestine>anterior intestine>stomach>mid intestine.

Cadmium accumulation and in vitro analysis of calcium and cadmium transport functions in the gastro-intestinal tract of trout following chronic dietary cadmium and calcium feeding.

Juvenile rainbow trout (Oncorhynchus mykiss) were fed diets made from Lumbriculus variegatus containing environmentally relevant concentrations of Cd (approximately 0.2 and 12 microg g(-1) dry wt) and/or Ca (1, 10, 20 and 60 mg g(-1) dry wt) for 4 weeks. Ten fish per treatment were removed weekly for tissue metal burden analysis.

Does dietary Ca protect against toxicity of a low dietborne Cd exposure to the rainbow trout?

We examined the toxicity of Cd, provided in a natural diet and at an environmentally relevant concentration ( approximately 12microgg(-1) dry wt.), to the juvenile rainbow trout (Oncorhynchus mykiss). In addition, we tested the protection by elevated dietary Ca against both the accumulation and toxicity of dietary Cd from this natural diet (background Ca approximately 1mgg(-1) dry wt.

Effects of using synthetic sea salts when measuring and modeling copper toxicity in saltwater toxicity tests.

Synthetic sea salts are often used to adjust the salinity of effluent, ambient, and laboratory water samples to perform toxicity tests with marine and estuarine species. The U.S.

Influence of water chemistry and natural organic matter on active and passive uptake of inorganic mercury by gills of rainbow trout (Oncorhynchus mykiss).

To distinguish physiologically regulated uptake from passive uptake of inorganic Hg in fish, rainbow trout (Oncorhynchus mykiss) were exposed to inorganic Hg (0.5, 1, or 2 microM total Hg) in ion-poor water with various treatments. Addition of ions to the water (mM concentrations of Ca, K, Cl) did not consistently alter Hg accumulation by trout gills, although there was a trend to higher Hg accumulation at higher ion concentrations.