The complete genome of clinical isolate URMC-2103A041 from human bacteremia was determined using the hybrid assembly of short- and long-read sequencing chemistry. The genome contains a 3.93 Mb chromosome, three circular plasmids, and one linear plasmid.
View Article and Find Full Text PDFCampylobacter ureolyticus is an emerging pathogen increasingly appreciated as a common cause of gastroenteritis and extra-intestinal infections in humans. Outside the setting of gastroenteritis, little work has been done to describe the genomic content and relatedness of the species, especially regarding clinical isolates. We reviewed the epidemiology of clinical C.
View Article and Find Full Text PDFThe genomes of two human monkeypox virus strains from recently reported cases in our local region that were associated with the 2022 global outbreak were sequenced. Genomes from clinical isolates provide valuable information for epidemiological tracking and analysis of strain evolution and can be especially important during the early phases of outbreaks.
View Article and Find Full Text PDFObjective: Bile and its individual components, mainly bile acids, are important for digestion and drive bacterial community dynamics in the upper gastrointestinal tract of chickens. However, specific responses to bile acids have been characterized in only a few commensal bacteria, and it is unclear how other members of the microbiota respond to biliary stress. Here, we used label-free LC-MS/MS to assess the proteomic response of a common inhabitant of the chicken small intestine, Turicibacter bilis MMM721, to 24 h of growth in anaerobic growth media supplemented with 0.
View Article and Find Full Text PDFInt J Syst Evol Microbiol
January 2022
Three novel, anaerobic, Gram-positive bacteria were isolated from the eggshell of two separate white leghorn chicken flocks and the ileum of a healthy pig, and designated MMM721, ISU324 and PIG517 respectively. Cells were pleomorphic and capable of forming long chains of rods or coccoid clusters. Phylogenetic analysis of the 16S rRNA gene sequences identified these strains to be within the genus , of which only one species, , has been formally described.
View Article and Find Full Text PDFMicrobiol Resour Announc
June 2020
MOL361 (DSM 14220) is the reference and type strain for the genus, commonly found in the intestinal tract of animal species. Long-read sequencing was performed on this strain to complement publicly available Illumina HiSeq-based data, producing a complete annotated genome sequence.
View Article and Find Full Text PDFBackground: The initial intestinal microbiota acquired from different sources has profound impacts on animal health and productivity. In modern poultry production practices, the source(s) of the establishing microbes and their overall contribution during development of gastrointestinal tract communities are still unclear. Using fertilized eggs from two independent sources, we assessed the impact of eggshell- and environmental-associated microbial communities on the successional processes and bacterial community structure throughout the intestinal tract of chickens for up to 6 weeks post-hatch.
View Article and Find Full Text PDFHere, we describe the complete genome sequence of sp. strain CE17, which was isolated during a screen for spore-forming anaerobic microorganisms that colonized the surface of chicken eggs.
View Article and Find Full Text PDFMicrobiol Resour Announc
January 2020
is the main cause of bacterial foodborne disease in humans, who are exposed mostly by consumption of contaminated poultry products. strain NADC 20827 was isolated from the feces of turkeys naturally colonized with spp. We present the complete annotated genome and plasmid sequences of strain NADC 20827.
View Article and Find Full Text PDFThe loss of antibiotics as a tool to improve feed efficiency in poultry production has increased the urgency to understand how the microbiota interacts with animals to impact productivity and health. Modulating and harnessing microbiota-host interactions is a promising way to promote poultry health and production efficiencies without antibiotics. In poultry, the microbiome is influenced by many host and external factors including host species, age, gut compartment, diet, and environmental exposure to microbes.
View Article and Find Full Text PDFMicrobiol Resour Announc
November 2018
A novel bacterium, Megasphaera stantonii (strain AJH120), was isolated from the cecum of a chicken during a screen for microorganisms using a brain heart infusion (BHI) medium. PacBio and Illumina MiSeq sequencing technologies were used to produce a single contiguous chromosome. The resulting genome sequence is 2,652,760 bp long with a 52.
View Article and Find Full Text PDFInt J Syst Evol Microbiol
November 2018
A novel mesophilic, anaerobic, Gram-stain-negative bacterium was isolated from the cecum of a healthy white leghorn chicken, and designated AJH120. Cells were coccoid or diplococcoid with an average size of 0.8-1.
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