E-cigarette aerosol induced cytotoxicity, DNA damages and late apoptosis in dynamically exposed A549 cells.

In this study, the acute toxicological impacts associated with electronic cigarettes consumption were determined using a novel dynamic exposure methodology. The methodology was deployed to test various e-cigarette generated aerosols in A549 cell cultures. The e-liquid chemical profiling was achieved using GC-MS analysis while toxicity of diluted e-liquids aerosols was reported using numerous cytotoxicity assays.

Evaluation of waterpipe smoke toxicity in C57BL/6 mice model.

Waterpipe smoking is a popular pastime worldwide with statistics pointing to an alarming increase in consumption. In the current paper, the evaluation of sub-chronic waterpipe smoke exposure was undertaken using C57BL/6 female mice using a dynamic exposure setting to emulate smoke exposure. Mice were daily subjected to either one (single exposure, SE) or two sessions (double exposure, DE) of waterpipe-generated smoke (two-apple flavor) for a period of two months.

Characterization and cytotoxicity assessment of nargile smoke using dynamic exposure.

Nargile (waterpipe) smoking has gained popularity in the Middle East and throughout the world. In this research, a new dynamic methodology was conceived. This methodology was deployed for direct assessment of cytotoxicity, genotoxicity, and apoptotic potential of smoke generated from a single nargile session.