Non-invasive Biofouling Monitoring to Assess Drinking Water Distribution System Performance.

Biofilms are endemic in drinking water distribution systems (DWDS), forming on all water and infrastructure interfaces. They can pose risks to water quality and hence consumers. Our understanding of these biofilms is limited, in a large part due to difficulties in sampling them without unacceptable disruption.

Assimilable organic carbon cycling within drinking water distribution systems.

A new conceptual model to describe and understand the role of assimilable organic carbon (AOC) within drinking water distribution systems is proposed. The impact of AOC on both drinking water biofilm and water quality was studied using bespoke pipe loop experimental facilities installed at three carefully selected operational water treatment works. Integrated physical, chemical and biological monitoring was undertaken that highlights the central role of biofilms in AOC cycling, forming the basis of the new conceptual model.

Application of enhanced assimilable organic carbon method across operational drinking water systems.

Assimilable organic carbon (AOC) is known to correlate with microbial growth, which can consequently degrade drinking water quality. Despite this, there is no standardised AOC test that can be applied to drinking water distribution systems (DWDS). Herein we report the development of a quick, robust AOC that incorporates known strains Pseudomonas fluorescens strain P-17 and Spirillum strain NOX, a higher inoculum volume and enumeration using flow cytometry to generate a quicker (total test time reduced from 14 to 8 days), robust method.

Biofilm Microbiome (Re)Growth Dynamics in Drinking Water Distribution Systems Are Impacted by Chlorine Concentration.

Biofilms are the dominant form of microbial loading (and organic material) within drinking water distribution systems (DWDS), yet our understanding of DWDS microbiomes is focused on the more easily accessible bulk-water. Disinfectant residuals are commonly provided to manage planktonic microbial activity in DWDS to safeguard water quality and public health, yet the impacts on the biofilm microbiome are largely unknown. We report results from a full-scale DWDS facility used to develop biofilms naturally, under one of three chlorine concentrations: Low, Medium, or High.

Characterisation of the physical composition and microbial community structure of biofilms within a model full-scale drinking water distribution system.

Within drinking water distribution systems (DWDS), microorganisms form multi-species biofilms on internal pipe surfaces. A matrix of extracellular polymeric substances (EPS) is produced by the attached community and provides structure and stability for the biofilm. If the EPS adhesive strength deteriorates or is overcome by external shear forces, biofilm is mobilised into the water potentially leading to degradation of water quality.

Methodological approaches for studying the microbial ecology of drinking water distribution systems.

The study of the microbial ecology of drinking water distribution systems (DWDS) has traditionally been based on culturing organisms from bulk water samples. The development and application of molecular methods has supplied new tools for examining the microbial diversity and activity of environmental samples, yielding new insights into the microbial community and its diversity within these engineered ecosystems. In this review, the currently available methods and emerging approaches for characterising microbial communities, including both planktonic and biofilm ways of life, are critically evaluated.

A new coupon design for simultaneous analysis of in situ microbial biofilm formation and community structure in drinking water distribution systems.

This study presents a new coupon sampling device that can be inserted directly into the pipes within water distribution systems (WDS), maintaining representative near wall pipe flow conditions and enabling simultaneous microscopy and DNA-based analysis of biofilms formed in situ. To evaluate this sampling device, fluorescent in situ hybridization (FISH) and denaturing gradient gel electrophoresis (DGGE) analyses were used to investigate changes in biofilms on replicate coupons within a non-sterile pilot-scale WDS. FISH analysis demonstrated increases in bacterial biofilm coverage of the coupon surface over time, while the DGGE analysis showed the development of increasingly complex biofilm communities, with time-specific clustering of these communities.