Publications by authors named "Joao S Patricio"
Article Synopsis
- Mitochondria play a key role in metabolic processes necessary for cell health and growth.
- Researchers studied how human cells react to mitochondrial dysfunction by examining metabolomics in fibroblasts from patients with mitochondrial disorders and cancer cells with disrupted electron transport chains (ETC).
- The findings showed that ETC defects lower de novo purine synthesis while increasing purine salvage, highlighting a shift in metabolism, particularly in lung cancer cells expressing high levels of the enzyme HPRT1, which is crucial for purine salvage.
- Targeting HPRT1 makes cancer cells more sensitive to ETC inhibition, revealing a new potential weakness in tumors with low oxidative metabolism.
Purpose: De novo lipogenesis (DNL) is critical for cell growth and maintenance, and acetyl-CoA precursors can be derived from different substrates. We developed a C NMR analysis of lipid extracts from cultured microglia cells administered with [U- C]glucose that informs overall lipogenic activity as well as the contribution of glucose to lipogenic acetyl-CoA.
Methods: BV-2 microglial cell line cultured with glucose and glutamine was provided with [U- C]glucose and unlabeled glutamine for 24 h and studied in either the presence or absence of lipopolysaccharide (LPS).