Publications by authors named "Joanna Stimpson"
Article Synopsis
- Inflammatory bowel diseases (IBD) are widespread but often show no symptoms early on, highlighting the need for effective, non-invasive diagnostic methods.
- This study proposes a technique to monitor IBD development by analyzing volatile organic compounds (VOCs) produced by gut microbiota using HS GC/MS during different inflammation stages in a rat model.
- Results showed significant changes in the metabolomic profile, particularly in short-chain fatty acids, during acute and remission phases of IBD, indicating potential biomarkers for early detection and monitoring of the disease.
Human exposure to DNA alkylating agents is poorly characterized, partly because only a limited range of specific alkyl DNA adducts have been quantified. The human DNA repair protein, -methylguanine -methyltransferase (MGMT), irreversibly transfers the alkyl group from DNA -alkylguanines (-alkGs) to an acceptor cysteine, allowing the simultaneous detection of multiple -alkG modifications in DNA by mass spectrometric analysis of the MGMT active site peptide (ASP). Recombinant MGMT was incubated with oligodeoxyribonucleotides (ODNs) containing different -alkGs, Temozolomide-methylated calf thymus DNA (Me-CT-DNA), or human colorectal DNA of known -MethylG (-MeG) levels.
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- Microorganisms and their hosts communicate through secreted proteins and small molecules, including volatile compounds (VOCs) like butyrate and propionate, which impact intestinal and immune functions.
- The study focuses on analyzing the VOC secretome of specific bacteria from the intestinal microbiota, particularly their outer membrane vesicles (OMVs), which may have effects beyond the gut.
- More than 60 VOCs were identified in bacterial culture media, including fatty acids and amino acid derivatives, with the first characterization of Bacteroides species' OMVs and their associated volatile compounds.