Hierarchical regulation of Burkholderia glumae type III secretion system by GluR response regulator and Lon protease.

Expression of type III secretion system (T3SS) genes, which are important for the virulence of phytopathogenic bacteria, is induced in the plant apoplastic environment or artificially amended growth conditions. Wild-type Burkholderia glumae BGR1, which causes rice panicle blight, induced a hypersensitive response (HR) in tobacco plants, whereas the T3SS genes were not significantly expressed in the commonly used hrp induction medium. T3SS gene expression in B.

Mutations in the Two-Component GluS-GluR Regulatory System Confer Resistance to β-Lactam Antibiotics in .

Bacteria have specific signaling systems to overcome selective pressure, such as exposure to antibiotics. The two-component system (TCS) plays an important role in the development of antibiotic resistance. Using the rice pathogen BGR1 as a model organism, we showed that the GluS (BGLU_1G13350) - GluR (BGLU_1G13360) TCS, consisting of a sensor kinase and response regulator, respectively, contributes to β-lactam resistance through a distinct mechanism.

Identification of a Genetically Linked but Functionally Independent Two-Component System Important for Cell Division of the Rice Pathogen .

Bacterial two-component regulatory systems control the expression of sets of genes to coordinate physiological functions in response to environmental cues. Here, we report a genetically linked but functionally unpaired two-component system (TCS) comprising the sensor kinase GluS (BGLU_1G13350) and the response regulator GluR (BGLU_1G13360), which is critical for cell division in the rice pathogen BGR1. The null mutant, unlike the mutant, formed filamentous cells in Lysogeny Broth medium and was sensitive to exposure to 42°C.