Electrospun Biodegradable Poly(L-lactic acid) Nanofiber Membranes as Highly Porous Oil Sorbent Nanomaterials.

Crude oil spills seriously harm the ocean environment and endanger the health of various animals and plants. In the present study, a totally biodegradable polymer, poly(L-lactic acid) (PLLA), was employed to fabricate highly porous oil absorbent nanofibrous materials by using a combination of electrospinning technique and subsequent acetone treatment. We systematically investigated how the electrospinning parameters affected formation of the porous structure of PLLA nanofibers and demonstrated that PLLA nanofibers with decreased and uniform diameter and improved porosity could be rapidly prepared by adjusting solution parameters and spinning parameters.

Prognostic role of pretreatment blood neutrophil-to-lymphocyte ratio in advanced cancer survivors: A systematic review and meta-analysis of 66 cohort studies.

Background: Neutrophil-to-lymphocyte ratio (NLR) is crucial for the incidence and mortality of various tumors. However, little is known on NLR and its association with prognosis in advanced tumors. Here we performed a meta-analysis to establish the prognostic significance of pretreatment blood NLR for advanced tumors.

Adsorption of uranium (VI) from aqueous solution using a novel graphene oxide-activated carbon felt composite.

Graphene oxide(GO)-activated carbon felt(ACF)(GO-ACF) composite was prepared by an electrophoretic deposition and subsequent thermal annealing. The structures of GO and GO-ACF were characterized by FT-IR, Raman spectra and XPS. The adsorption capacities for U(VI) from aqueous solution of ACF and GO-ACF were compared.

[Screening and cloning gene of hepatocyte protein interacting with hepatitis C virus core protein].

Objective: To clone the unknown gene of hepatocyte protein interacting with hepatitis C virus core protein.

Methods: Using the yeast dual hybrid system 3, bait plasmids of hepatitis C virus core were constructed. After identifying hepatitis C virus core protein that could stably expressed in AH109 yeast strains, we performed yeast two hybrid by mating AH109 with Y187 that transformed with liver cDNA library plasmids pACT2 and then plated on quadrople dropout (QDO) medium and assayed for alpha-gal activity.

[Inducible expression of non-structural protein 3 of hepatitis C virus in E. coli].

Background: To express recombinant non-structural protein 3 of hepatitis C virus (HCV) in E. coli.

Methods: The non-structural 3 (NS3) region DNA fragment of HCV was amplified by polymerase chain reaction (PCR) and inserted into inducible proeukaryotic expressive vector pET 30C(+)at Bam H1/EcoR1 sites.

[Construction and expression of humanized anti-HBsAg scFv targeting interferon-alpha in escherichia coli].

Objective: To develop a bacteria expression system to produce the fusion protein of humanized anti-HBsAg scFV and interferon-alpha.

Methods: The expression vector was constructed after cleaving the plasmids harboring the humanized anti-HBsAg scFv and interferon alpha respectively and ligating to linearized pET22b subsequence. The expression of fusion protein in E.

Primary porcine hepatocytes with portal vein serum cultured on microcarriers or in spheroidal aggregates.

AIM:To develop a culture mode providing durable biomaterials with high yields and activities used in bioartificial liver.METHODS:Hepatocytes were isolated from a whole pig liver by Seglen s method of orthotopic perfusion with collagenase. In culture on microcarriers, primary porcine hepatocytes were inoculated at a concentration of 5center dot10(7)/mL into the static culture systems containing 2g/L Cytodex-3, then supplemented with 100mL/L fetal calf serum (FCS) or 100mL/L porcine portal vein serum (PPVS) respectively.

Cultivation of human liver cell lines with microcarriers acting as biological materials of bioartificial liver.

AIM:To improve the cultivation efficiency and yield of human liver cell line CL-1.METHODS:High-density cultivation of CL-1 on microcarriers was carried out with periodic observation of their growth and proliferation. The specific functions of human liver cell were also determined.