Benzylurea Protects hPDLFs Against LPS-Induced Mitochondrial Dysfunction Through MTCH2.

Objective: The aim of this study is to explore the mechanism of benzylurea in the inflammatory injury of human periodontal ligament fibroblasts (hPDLFs).

Methods: An inflammation model of hPDLFs was established using LPS. Nuclear transport of nuclear transcription factor-κB (NF-κB), secretion of cytokines, and the morphology and distribution of F-actin were determined.

Effects of Ward Night Noise Management in the Context of Enhanced Recovery After Surgery on Postoperative Sleep Quality, Anxiety, and Hormone Levels of Thoracic Surgery Patients with Lung Cancer.

Objective: This study aimed to analyze the effects of ward night noise management in the context of enhanced recovery after surgery (ERAS) on postoperative sleep quality and anxiety of thoracic surgery patients with lung cancer.

Methods: This retrospective analysis included 118 patients with lung cancer who underwent thoracic surgery (from January 2020 to December 2021). The patients were categorized into the control and observation groups, which comprised 63 and 55 cases, respectively, on the basis of perioperative management plans.

The effectiveness of intravenous zoledronic acid in elderly patients with osteoporosis after rotator cuff repair: a retrospective study.

The aim of this study was to investigate the effect of zoledronic acid (ZA) on postoperative healing and functional rehabilitation in osteoporotic patients with rotator cuff (RC) injury. 96 Patients were divided into three groups according to bone mineral density and ZA use (Group A: normal BMD; Group B: osteoporosis and intravenous ZA use; Group C: osteoporosis, without ZA use). Radiologic, functional and Serological outcomes were evaluated 6 months after surgery.

Accurate and High-Resolution Particle Mass Measurement Using a Peak Filtering Algorithm.

Charge detection quadrupole ion trap mass spectrometry (CD-QIT MS) is an effective way of achieving the mass analysis of microparticles with ultrahigh mass. However, its mass accuracy and resolution are still poor. To enhance the performance of CD-QIT MS, the resolution of each peak in the mass spectra resulting from an individual particle was assessed, and a peak filtering algorithm that can filter out particle adducts and clusters with a lower was proposed.

Maturity degree assessment of hospital ward system using integrated fuzzy AHP-TOPSIS model.

Background: The hospital ward system is the core service unit of a hospital and an important aspect of hospital management. The maturity of the hospital ward system represents the level of development and improvement in ward management and services. In order to improve the quality of hospital services, it is significant to assess the maturity of the ward system.

In situ analysis of metabolic changes under hypoxic-ischemic encephalopathy via MALDI mass spectrometry imaging.

Hypoxic-ischemic encephalopathy (HIE) is a leading cause of neurological disability and even more serious fetal or neonatal asphyxia death. As the therapeutic time window is limited and timely intervention could have a better prognosis, elucidating the mechanisms underlying HIE and developing novel therapeutic strategies is of great importance. In the present study, 1, 5-Diaminonaphthalene hydrochloride (1, 5-DANHCl) assisted laser desorption/ionization mass spectrometry imaging (MALDI MSI) was applied to the neonatal rat model of HIE to investigate metabolic changes during hypoxic-ischemic period.

Biofluid Metabolic Profiling for Lung Cancer Screening via Reactive Matrix-Assisted Laser Desorption Ionization Mass Spectrometry.

Lung cancer (LC) has the highest mortality rate among various cancer diseases. Developing an early screening method for LC with high classification accuracy is essential. Herein, 2-hydrazinoquinoline (2-HQ) is utilized as a dual-mode reactive matrix for metabolic fingerprint analysis and LC screening via matrix-assisted laser desorption ionization mass spectrometry (MALDI-MS).

Nasal Alar and Scalp Metastases From Lung Carcinoma: A Case Report and Literature Review.

Both nasal alar and scalp metastases from lung carcinoma are exceedingly rare. Herein, we report a case of an elderly male smoker who had left lung carcinoma and underwent radical resection. Seven months later, masses began to appear on his nasal alar and parietal scalp which gradually increased in size.

Interfacial and rheological properties of long-lived foams stabilized by rice proteins complexed to transition metal ions in the presence of alkyl polyglycoside.

Hypothesis: Transition-metal coordination complexes are hopeful to make advanced structural materials, since the metal-coordination bonds, unlike typical covalent bonds, can regenerate after rupture, allowing for dynamic, tunable, and reversible mechanical characteristics. Integration of metal-coordinate crosslinking in foam material has rarely been reported.

Experiments: We developed the hydrolyzed rice proteins (HRP) as the building block for amphiphilic transition-metal coordination complexes that could be used to make long-lived foams with high yield stress.

High Speed Mass Measurement of a Single Metal-Organic Framework Nanocrystal in a Paul Trap.

Mass spectrometry (MS) has emerged as an excellent tool for the characterization of metal-organic frameworks (MOFs) based on the characteristic metal ions and organic ligands. Mass measurement of intact MOF nanocrystals, however, remains a challenge for MS technology. Here, we reported the development of a probe particles based charge detection-quadrupole ion trap mass spectrometry (probe CD-QIT MS) method, where charge detection and mass measurement of a single MOF nanocrystal were achieved under the assistance of probe particles of micrometer size.

Effect of static magnetic field extended supercooling preservation on beef quality.

Supercooling can preserve beef without freezing damage, whereas maintaining the supercooled state is difficult. An innovative method of static magnetic field extended supercooling (SM-ES) was proposed to maintain the non-frozen state of beef. Effect of SM-ES (-4 °C + SMF) compared with refrigerated (4 °C), slow-frozen (-4 °C) and frozen (-18 °C) treatment on beef quality was investigated.

Collagen Family Genes Associated with Risk of Recurrence after Radiation Therapy for Vestibular Schwannoma and Pan-Cancer Analysis.

Background: The safety of radiotherapy techniques in the treatment of vestibular schwannoma (VS) shows a high rate of tumor control with few side effects. Neuropeptide Y (NPY) may have a potential relevance to the recurrence of VS. Further research is still needed on the key genes that determine the sensitivity of VS to radiation therapy.

[Effects of different dosages of oral glucocorticoids on short-term recovery and recurrence of eosinophilic chronic rhinosinusitis with nasal polyps].

To analyze the effects of different dosages of oral glucocorticoids on short-term recovery and recurrence in patients with eosinophilic chronic rhinosinusitis with nasal polyps（ECRSwNP）. 150 patients with ECRSwNP who underwent functional endoscopic sinus surgery for the first time after the failure of maximum drug therapy were recruited. They were randomly divided into group A, B and C, and their baseline demographic and clinical characteristics were recorded.

[Clinical analysis and surgical discussion of juvenile ossifying fibroma in nasal root].

To analyze the clinical features of juvenile ossifying fibroma in nasal root and to compare different surgical methods. Seven cases of juvenile ossifying fibroma occurring in the nasal root were treated via surgery, 5 cases were resected under nasal endoscopy guided by Image Guidance System-based electromagnetic navigation, and 2 cases were resected by lateral nasal butterfly incision. Postoperative follow-up included endoscopy and CT scan of the sinuses.

MALDI-TOF/TOF tandem mass spectrometry imaging reveals non-uniform distribution of disaccharide isomers in plant tissues.

Mass spectrometry imaging (MSI) is a powerful technique for investigating the biomolecular locations within tissues. However, the isomeric compounds are rarely distinguished due to inability of MSI to differentiate isomers in the probing area. Coupling tandem mass spectrometry with MSI can facilitate differentiating isomeric compounds.

Laser cleavable probes for multiplexed glycan detection by single cell mass spectrometry.

Glycans binding on the cell surface through glycosylation play a key role in controlling various cellular processes, and glycan analysis at a single-cell level is necessary to study cellular heterogeneity and diagnose diseases in the early stage. Herein, we synthesized a series of laser cleavable probes, which could sensitively detect glycans on single cells and tissues by laser desorption ionization mass spectrometry (LDI-MS). This multiplexed and quantitative glycan detection was applied to evaluate the alterations of four types of glycans on breast cancer cells and drug-resistant cancer cells at a single-cell level, indicating that drug resistance may be related to the upregulation of glycan with a β-d-galactoside (Galβ) group and Neu5Aca2-6Gal(NAc)-R.

Circulating miR-214-3p predicts nasopharyngeal carcinoma recurrence or metastasis.

Background: Due to the remarkably stable form in the bloodstream, circulating microRNAs (miRNAs) are indicated as promising novel minimally invasive biomarkers in many cancers. However, available data of miRNAs in nasopharyngeal carcinoma (NPC) are relatively limited.

Methods: Based on the GEO database and previous published reports, 21 dysregulated miRNAs were selected for screening via microarray analysis (20 NPC samples vs 10 controls).

Correction: Laser cleavable probes for multiplexed glycan detection by single cell mass spectrometry.

[This corrects the article DOI: 10.1039/C9SC03912K.].

Enhancing surface-assisted laser desorption ionization mass spectrometry performance by integrating plasmonic hot-electron transfer effect through surface modification.

Surface-assisted laser desorption ionization mass spectrometry (SALDI MS) performances were enhanced by modifying surfaces to include a plasmonic hot-electron transfer effect. After modification, the core material's specificity was not only maintained but also enhanced. The MS imaging of ischemic tissues with the modified material indicated some sulfatide changes, which have been barely reported.

Hot electron transfer promotes ion production in plasmonic metal nanostructure assisted laser desorption ionization mass spectrometry.

Plasmonic metal nanostructures have been used as effective materials for enhancing the laser desorption ionization (LDI) efficiency in mass spectrometry analysis. But the ionization mechanism and the origin of initial charges have not been fully understood yet. Here we provided experimental evidence showing that the hot electron transfer in localized surface plasmon resonance (LSPR) plays a key role in ionizing molecules during the LDI process.

N-Phenyl-2-naphthylamine as a Novel MALDI Matrix for Analysis and in Situ Imaging of Small Molecules.

Due to its strong ultraviolet absorption, low background interference in the small molecular range, and salt tolerance capacity, N-phenyl-2-naphthylamine (PNA) was developed as a novel matrix in the present study for analysis and imaging of small molecules by matrix-assisted laser desorption/ionization mass spectrometry time-of-fight (MALDI-TOF MS). The newly developed matrix displayed good performance in analysis of a wide range of small-molecule metabolites including free fatty acids, amino acids, peptides, antioxidants, and phospholipids. In addition, PNA-assisted LDI MS imaging of small molecules in brain tissue of rats subjected to middle cerebral artery occlusion (MCAO) revealed unique distributions and changes of 89 small-molecule metabolites including amino acids, antioxidants, free fatty acids, phospholipids, and sphingolipids in brain tissue 24 h postsurgery.

Hexagonal boron nitride nanosheets as a multifunctional background-free matrix to detect small molecules and complicated samples by MALDI mass spectrometry.

Here we report the innovation of using hexagonal boron nitride (h-BN) nanosheets as the first background-free matrix among the full laser pulse energy range for the analysis and imaging of small molecule metabolites and as an adsorbent for the enrichment of samples. Due to its totally free background and high efficiency as an adsorbent and matrix, h-BN has promising prospects in metabolomics, MALDI imaging, and environmental analysis.

Mass Spectrometry Imaging of Kidney Tissue Sections of Rat Subjected to Unilateral Ureteral Obstruction.

Chronic kidney disease (CKD) poses a serious threat to the quality of human life and health with an increasing incidence worldwide. Renal fibrosis is closely related to CKD and regarded as the final common pathophysiological pathway in most cases of end-stage renal diseases. Elucidating the mechanisms underlying renal fibrosis and developing novel therapeutic strategies are of great importance.