Zoledronic Acid Regulates Osteoclasts via miR-483-5p in the BRONJ.

Objectives: Bisphosphonate-related osteonecrosis of the jaw (BRONJ) is a severe complication of bisphosphonate therapy, with unclear mechanisms. This study investigates the regulatory impact of zoledronic acid (ZOL) on osteoclasts and microRNA (miRNA) expression.

Materials And Methods: Raw264.

Nutrient allocation patterns in different aboveground organs at different reproductive stages of four introduced species in a common garden in northwestern China.

Introduction: The species is a typical shrub with assimilative branches (ABs) in arid regions in Central Asia. The nutrient distribution patterns at different reproductive stages are of great significance for further understanding the ecological adaptation and survival strategies of plants.

Methods: In the present study, a common garden experiment was employed to avoid interference by environmental heterogeneity.

Intermediate-regulated dynamic restructuring at Ag-Cu biphasic interface enables selective CO electroreduction to C fuels.

A bimetallic heterostructure has been shown effective to enhance the multi-carbon (C) product selectivity in CO electroreduction. Clarifying the interfacial structure under electrolysis and its decisive role in the pathway selection are crucial, yet challenging. Here, we conceive a well-defined Ag-Cu biphasic heterostructure to understand the interfacial structure-steered product selectivity: The Cu-rich interface prefers ethylene, while the dominant product switch to alcohols with an increasing Ag fraction, and finally to CO as Ag occupying the main surface.

Unraveling Key Amino Acid Residues Crucial for PxGSTs1 Conferring Benzoylurea Insecticide Resistance in .

The widespread use of benzoylurea insecticides (BUs) has led to significant resistance issues in various agricultural pests. Previous studies have demonstrated that the overexpression of sigma glutathione S-transferase 1 (PxGSTs1) can confer resistance to novaluron in ; however, the underlying molecular mechanism remains unclear. This study investigates the role of glutathione S-transferase PxGSTs1 in mediating resistance to BUs in .

dmTGS: Precise Targeted Enrichment Long-Read Sequencing Panel for Tandem Repeat Detection.

Background: Tandem repeats (TRs) are abundant in the human genome and associated with repeat expansion disorders. Our study aimed to develop a tandem repeat panel utilizing targeted long-read sequencing to evaluate known TRs associated with these disorders and assess its clinical utility.

Methods: We developed a targeted long-read sequencing panel for 70 TR loci, termed dynamic mutation third-generation sequencing (dmTGS), using the PacBio Sequel II platform.

A Homoplasmic MT-TV Mutation Associated with Mitochondrial Inheritance of Hereditary Spastic Paraplegia.

Background: Hereditary spastic paraplegia (HSP) is characterized by progressive lower limb weakness and spasticity, with unknown genetic cause in many cases.

Objectives: To identify novel genetic causes of HSP.

Methods: Phenotypic characterization, genetic screening, transcriptome sequencing, and peroneal nerve biopsy were conducted in a Chinese HSP family.

Dynamic Adaptation of Active Site Driven by Dual-side Adsorption in Single-Atomic Catalysts During CO Electroreduction.

Single-atom iron embedded in N-doped carbon (Fe-N-C) is among the most representative single-atomic catalysts (SACs) for electrochemical CO reduction reaction (CORR). Despite the simplicity of the active site, the CO-to-CO mechanism on Fe-N-C remains controversial. Firstly, there is a long debate regarding the rate-determining step (RDS) of the reactions.

HPV-YAP1 oncogenic alliance drives malignant transformation of fallopian tube epithelial cells.

High grade serous ovarian carcinoma (HGSOC) is the most common and aggressive ovarian malignancy. Accumulating evidence indicates that HGSOC may originate from human fallopian tube epithelial cells (FTECs), although the exact pathogen(s) and/or molecular mechanism underlying the malignant transformation of FTECs is unclear. Here we show that human papillomavirus (HPV), which could reach FTECs via retrograde menstruation or sperm-carrying, interacts with the yes-associated protein 1 (YAP1) to drive the malignant transformation of FTECs.

Manipulation of Oxygen Species on an Antimony-Modified Copper Surface to Tune the Product Selectivity in CO Electroreduction.

Rational regulation of the electrochemical CO reduction reaction (CORR) pathway to produce desired products is particularly interesting, yet designing economical and robust catalysts is crucial. Here, we report an antimony-modified copper (CuSb) catalyst capable of selectively producing both CO and multicarbon (C) products in the CORR. At a current density of 0.

The effect of vitamin D2 on lipid profile, anthropometric indices, blood pressure, and inflammatory and glycemic biomarkers in humans: A systematic review and meta-analysis of randomized controlled trials.

Background And Aim: Even though the role of D2 (ergocalciferol) on cardiovascular disease risk components has been studied, conflicting results have been reported. Moreover, no single study has studied all these parameters and the role of vitamin D2 individually has not been assessed; hence, this systematic review and meta-analysis of randomized controlled trials was conducted to assess the effect of vitamin D2 supplementation on lipid profile, anthropometric indices, blood pressure, and inflammatory and glycemic biomarkers in humans.

Methods: Web of Science, Scopus, PubMed/Medline, and Embase were searched from database inception to July 2024, and the random effects model, according to the DerSimonian and Laird method, was used to generate combined estimates of the intervention's effect on the outcomes.

Elucidating the detoxification efficacy of Periplaneta americana delta glutathione S-transferase 1 (PaGSTd1) against organophosphates.

As an important class of detoxifying enzymes, glutathione S-transferases (GSTs) are pivotal in decreasing insecticide toxicity to insects. Periplaneta americana GSTd1 (PaGSTd1) has been verified as a key enzyme in detoxifying pyrethroid insecticides, but its detoxification capability against a broader spectrum of insecticides has never been investigated. It is revealed that PaGSTd1 expression showed a rapid and significant increase upon exposure to various insecticides (organophosphates, neonicotinoids, and fipronil).

Cu/CuO/Graphdiyne Tandem Catalyst for Efficient Electrocatalytic Nitrate Reduction to Ammonia.

The electrocatalytic reduction reaction of nitrate (NO ) to ammonia (NH) is a feasible way to achieve artificial nitrogen cycle. However, the low yield rate and poor selectivity toward NH product is a technical challenge. Here a graphdiyne (GDY)-based tandem catalyst featuring Cu/CuO nanoparticles anchored to GDY support (termed Cu/CuO/GDY) for efficient electrocatalytic NO reduction is presented.

[H3K27 acetylation promotes lncRNA OIP5-AS1 transcription and induces apoptosis of nasal epithelial cells in allergic rhinitis through up-regulation of TLR4].

Objective To investigate the effect of lysine 27 residue of histone H3 (H3K27) acetylation modification on the transcriptional promotion of long noncoding RNA OPA interacting protein 5-antisense RNA 1 (lncRNA OIP5-AS1) and apoptosis of nasal epithelial cells (NECs) in allergic rhinitis (AR) via regulating Toll-like receptor 4 (TLR4). Methods Interleukin-13 (IL-13) was used to treat NECs to establish an AR cell model. Real-time quantitative PCR was utilized to detect the expressions of OIP5-AS1 and TLR4 in nasal mucosal tissues of AR patients and in the in vitro cell model.

Nanoparticles for efficient drug delivery and drug resistance in glioma: New perspectives.

Gliomas are the most common primary tumors of the central nervous system, with glioblastoma multiforme (GBM) having the highest incidence, and their therapeutic efficacy depends primarily on the extent of surgical resection and the efficacy of postoperative chemotherapy. The role of the intracranial blood-brain barrier and the occurrence of the drug-resistant gene O6-methylguanine-DNA methyltransferase have greatly limited the efficacy of chemotherapeutic agents in patients with GBM and made it difficult to achieve the expected clinical response. In recent years, the rapid development of nanotechnology has brought new hope for the treatment of tumors.

Fast Continual Multi-View Clustering With Incomplete Views.

Multi-view clustering (MVC) has attracted broad attention due to its capacity to exploit consistent and complementary information across views. This paper focuses on a challenging issue in MVC called the incomplete continual data problem (ICDP). Specifically, most existing algorithms assume that views are available in advance and overlook the scenarios where data observations of views are accumulated over time.

On the Consistency and Large-Scale Extension of Multiple Kernel Clustering.

Existing multiple kernel clustering (MKC) algorithms have two ubiquitous problems. From the theoretical perspective, most MKC algorithms lack sufficient theoretical analysis, especially the consistency of learned parameters, such as the kernel weights. From the practical perspective, the high complexity makes MKC unable to handle large-scale datasets.

B7-H3 suppresses CD8 T cell immunologic function through reprogramming glycolytic metabolism.

Malignant neoplasms pose a formidable threat to human well-being. Prior studies have documented the extensive expression of B7 homolog 3 (B7-H3 or CD276) across various tumors, affecting glucose metabolism. Yet, the link between metabolic modulation and immune responses remains largely unexplored.

One-Step Multi-View Clustering With Diverse Representation.

Multi-View clustering has attracted broad attention due to its capacity to utilize consistent and complementary information among views. Although tremendous progress has been made recently, most existing methods undergo high complexity, preventing them from being applied to large-scale tasks. Multi-View clustering via matrix factorization is a representative to address this issue.

Identifying the Key Role of General Odorant Binding Protein 2 in Perceiving a Larval Attractant, (,)-2,6-Farnesol.

There is currently a lack of effective olfaction-based techniques to control diamondback moth (DBM) larvae. Identifying behaviorally active odorants for DBM larvae and exploring their recognition mechanisms can provide insights into olfaction-based larval control strategies. Through the two-choice assay, (,)-2,6-farnesol (farnesol) was identified as a compound exhibiting significant attractant activity toward DBM larvae, achieving an attraction index of 0.

Efficient C-N coupling for urea electrosynthesis on defective CoO with dual-functional sites.

Urea electrosynthesis under ambient conditions is emerging as a promising alternative to conventional synthetic protocols. However, the weak binding of reactants/intermediates on the catalyst surface induces multiple competing pathways, hindering efficient urea production. Herein, we report the synthesis of defective CoO catalysts that integrate dual-functional sites for urea production from CO and nitrite.

Key Contributions of the Overexpressed Sigma Glutathione -Transferase 1 Gene () in the Resistance Evolution to Multiple Insecticides.

The overexpression of insect detoxification enzymes is a typical adaptive evolutionary strategy for insects to cope with insecticide pressure. In this study, we identified a glutathione -transferase (GST) gene, , that exhibited pronounced expression in the field-resistant population of . By using RNAi (RNA interference), the transgenic fly models, and quantitative real-time polymerase chain reaction (RT-qPCR) methods, we confirmed that the augmented expression of mediates the resistance of to various types of insecticides, including chlorantraniliprole, novaluron, λ-cyhalothrin, and abamectin.

YBX1 Underwent Phase Separation into Stress Granules Stimulated by Ionizing Radiation.

Stress granules (SGs) are formed through liquid-liquid phase separation (LLPS), in response to external stimuli. YBX1, an integral component of SGs, plays a crucial role in tumor progression and cellular stress response. This study aims to elucidate the mechanisms and specific biological implications of YBX1 in SG formation, along with the identification of key regions and interacting proteins.

A biosensor for D-2-hydroxyglutarate in frozen sections and intraoperative assessment of IDH mutation status.

The oncometabolite D-2-hydroxyglutarate (D-2-HG) has emerged as a valuable biomarker in tumors with isocitrate dehydrogenase (IDH) mutations. Efficient detection methods are required and rapid intraoperative determination of D-2-HG remains a huge challenge. Herein, D-2-HG dehydrogenase from Achromobacter xylosoxidans (AX-D2HGDH) was found to have high substrate specificity.