Biosynthesis of L-5-methyltetrahydrofolate by genetically engineered Escherichia coli.

L-5-Methyltetrahydrofolate (L-5-MTHF) is the only biologically active form of folate in the human body. Production of L-5-MTHF by using microbes is an emerging consideration for green synthesis. However, microbes naturally produce only a small amount of L-5-MTHF.

A Cytoplasmic NAD(P)H-Dependent Polysulfide Reductase with Thiosulfate Reductase Activity from the Hyperthermophilic Bacterium Thermotoga maritima.

Thermotoga maritima is an anaerobic hyperthermophilic bacterium that efficiently produces H by fermenting carbohydrates. High concentration of H inhibits the growth of T. maritima, and S could eliminate the inhibition and stimulate the growth through its reduction.

A Heterodimeric Reduced-Ferredoxin-Dependent Methylenetetrahydrofolate Reductase from Syngas-Fermenting Clostridium ljungdahlii.

The strict anaerobe Clostridium ljungdahlii can ferment CO or H/CO via the Wood-Ljungdahl pathway to acetate, ethanol, and 2,3-butanediol. This ability has attracted considerable interest, since it can be used for syngas fermentation to produce biofuels and biochemicals. However, the key enzyme methylenetetrahydrofolate reductase (MTHFR) in the Wood-Ljungdahl pathway of the strain has not been characterized, and its physiological electron donor is unclear.

Behavioural Problems Amongst Pre-School Children in Chongqing, China: Current Situation and Influencing Factors.

Introduction: Behavioural problems in pre-school children are closely related to their mental health. Such problems include attention deficit, personality disorder, overdependency, poor adaptability and conduct problems.

Methods: From December 2018 to January 2019, we conducted a cross-sectional survey of parents of pre-schoolers.

Parental Influence in Forming Preschool Children's Eating Behaviors-A Cross-Sectional Survey in Chongqing, China.

Children's eating habits are closely related to their health problems and the outlook for children's nutritional statuses appears poor. A cross-sectional survey was conducted among parents of preschool children from December 2018 to January 2019. Sixteen representative kindergartens in 6 districts of Chongqing, China, were included in the study.

Distribution, Evolution, Catalytic Mechanism, and Physiological Functions of the Flavin-Based Electron-Bifurcating NADH-Dependent Reduced Ferredoxin: NADP Oxidoreductase.

NADH-dependent reduced ferredoxin:NADP oxidoreductase (Nfn) is an electron-bifurcating enzyme first discovered in the strict anaerobes and . , Nfn catalyzes the endergonic reduction of NADP with NADH coupled to the exergonic reduction of NADP with reduced ferredoxin. Most Nfn homologs consist of two subunits, although in certain species Nfn homologs are fused.

Green route to synthesis of valuable chemical 6-hydroxynicotine from nicotine in tobacco wastes using genetically engineered S33.

Background: Tobacco is widely planted as an important nonfood economic crop throughout the world, and large amounts of tobacco wastes are generated during the tobacco manufacturing process. Tobacco and its wastes contain high nicotine content. This issue has become a major concern for health and environments due to its toxicity and complex physiological effects.