Light calcium carbonate improves pullulan biosynthesis by Aureobasidium pullulans under high concentration of sugar.

The effects of light calcium carbonate (CaCO) on pullulan biosynthesis by Aureobasidium pullulans NCPS2016 were investigated. Light CaCO enhanced pullulan production by 12.4 % when added to the low concentration of fructose broth compared with KHPO.

High-level production of pullulan from high concentration of glucose by mutagenesis and adaptive laboratory evolution of Aureobasidium pullulans.

The cost of carbon sources and the low efficiency of the fermentation titer limit the industrial application of pullulan. In this study, a hypertonic-tolerant strain with efficient utilization of glucose was obtained using a double strategy. Initially, a strain for efficient synthesis of pullulan from glucose was generated by mutagenesis.

Identification of Lipopeptide Iturin A Produced by NCPSJ7 and Its Antifungal Activities against f. sp. .

NCPSJ7 showed potential fungicidal activities for the effective control of fungal infection. From the PCR test, the key genes (, , , , , and ) were detected in NCPSJ7. These genes were closely related to the lipopeptides (LPs) synthesis.

Divergent Conversion of Double Isocyanides with Alkenyl Bromide to Polysubstituted Pyrroles and 4-Imino-4,5-dihydropyrrolo[3,4-]pyrrol-6(1)-one Derivatives by Pd-Catalyzed Tandem Cyclization Reactions.

Herein a novel and concise approach to pyrrole skeletons via Pd-catalyzed tandem cyclization reactions is investigated. The substrates for the transformation could be readily prepared by phosphoric acid-catalyzed Ugi reactions with available starting materials. In this strategy, two isocyanides participate in sequential isocyanide insertion reactions, and the chemoselectivity of the products is regulated by the steric hindrance of the isocyanide.

Eco-Friendly and Facile Synthesis of Antioxidant, Antibacterial and Anticancer Dihydromyricetin-Mediated Silver Nanoparticles.

Background: Dihydromyricetin (DMY), a natural flavonoid, has reportedly antibacterial, antioxidant, anticancer and other properties. In the present study, DMY was used as a reducing agent and stabilizer to synthesize silver nanoparticles (AgNPs), and the optimal conditions for its synthesis were studied. The DMY-AgNPs were investigated for their DPPH scavenging properties and their potential against human pathogenic and food-borne bacteria viz.

Green and Facile Synthesis and Antioxidant and Antibacterial Evaluation of Dietary Myricetin-Mediated Silver Nanoparticles.

Myricetin (MY) is a dietary flavonoid which exhibits a wide spectrum of biological properties, viz., antibacterial, antioxidant, anticancer, and so forth. The lower solubility in aqueous medium and hence lesser bioavailability of MY limits the use of such dietary flavonoids in further in vivo research.

Application of antagonist NCPSJ7 against in postharvest Red Globe grapes.

We investigated the effects and possible mechanisms of NCPSJ7 against the gray mold caused by in the postharvest Red Globe grapes. The disease incidence, lesion diameter, decay index, and some resistance-related enzymes were evaluated. The antioxidant capacity of grape treated with 1 × 10 CFU/ml alone and combined with 1 × 10 CFU/ml NCPSJ7 was also determined.

Fast atrazine degradation by the mixed cultures enriched from activated sludge and analysis of their microbial community succession.

In this study, fast atrazine degradation by the mixed bacterial cultures from sewage sludge was investigated. The acquired activated cultures showed great capability in atrazine degradation. The biodegradation process was well fitted into a pseudo-first reaction kinetic model.

Screening and Identifying Antioxidative Components in Ginkgo biloba Pollen by DPPH-HPLC-PAD Coupled with HPLC-ESI-MS2.

The Ginkgo biloba is one of ancient trees that exists from billions of years ago, its leaf and nut are used as herbs and foods in China, while so far its pollen does not have any application except pollination. In order to evaluate the antioxidant activity of Ginkgo biloba pollen, and rapidly screen its antioxidative components, the 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging ability, total flavonoid, total phenol, and proanthocyanidin of Ginkgo biloba pollen were determined and compared with those of Ginkgo biloba leaf and nut, and the off-line DPPH-HPLC-PAD and HPLC-ESI-MS2 were applied for screening and identifying the antioxidant flavonoids in Ginkgo biloba pollen. The results showed that the DPPH scavenging ability of Ginkgo biloba pollen was much higher than Ginkgo biloba nut, but lower than Ginkgo biloba leaf, while the total content of flavonoid in Ginkgo biloba pollen was approximately 4.

Screening natural antioxidants in peanut shell using DPPH-HPLC-DAD-TOF/MS methods.

Peanut shell, a byproduct in oil production, is rich in natural antioxidants. Here, a rapid and efficient method using DPPH-HPLC-DAD-TOF/MS was used for the first time to screen antioxidants in peanut shell. The method is based on the hypothesis that upon reaction with 1, 1-diphenyl-2-picrylhydrazyl (DPPH), the peak areas of compounds with potential antioxidant activities in the HPLC chromatogram will be significantly reduced or disappeared, and the identity confirmation could be achieved by HPLC-DAD-TOF/MS technique.