Genetic breakthroughs in the - interactions.

(Lib.) de Bary is a highly destructive fungal pathogen that seriously damages the yield and quality of worldwide. The complex interaction between the and system has presented significant challenges in researching rapeseed defense strategies.

Extracellular enzyme stoichiometry reveals carbon and nitrogen limitations closely linked to bacterial communities in China's largest saline lake.

Saline lakes possess substantial carbon storage and play essential roles in global carbon cycling. Benthic microorganisms mine and decompose sediment organic matter via extracellular enzymes to acquire limiting nutrients and thus meet their element budgets, which ultimately causes variations in sediment carbon storage. However, current knowledge about microbial nutrient limitation and the associated organic carbon changes especially in saline lake remains elusive.

[Vertical Distribution Characteristics and Influencing Factors of Phytoplankton Community Structure in Qiandao Lake].

Clear vertical variations in phytoplankton community structure are usually observed in deep-water lakes and reservoirs, which is one of the key components of water quality and ecosystem functioning. However, the vertical patterns and ecological drivers of phytoplankton communities in deep-water lakes and reservoirs are still understudied. In this study, we took Qiandao Lake, a deep-water reservoir, as an example to reveal the vertical distribution characteristics of phytoplankton communities and its influencing factors by investigating phytoplankton community structure and the associated water quality index at 12 sites across the whole lake in two seasons (spring and autumn).

Prognostic value of aspartyl (asparaginyl)-beta-hydroxylase/humbug expression in non-small cell lung carcinoma.

Despite improvements in the detection and use of biomarkers, including epidermal growth factor receptor, ERCC1, and p16, the 5-year survival rate with non-small cell lung cancer remains at 15%. This suggests that additional biomarkers are needed to better prognosticate clinical course and guide therapeutic approaches. Previous studies showed that increased levels of aspartyl (asparaginyl)-beta-hydroxylase and a highly related molecule, humbug, correlate with clinical course and survival with hepatic, biliary, pancreatic, and colon carcinomas.

Gene expression in mouse brain following chronic hypoxia: role of sarcospan in glial cell death.

Hypoxia is a hallmark of respiratory, neurological, or hematological diseases as well as life at high altitude. For example, chronic constant hypoxia (CCH) occurs in chronic lung diseases or at high altitude, whereas chronic intermittent hypoxia (CIH) occurs in diseases such as sleep apnea or sickle cell disease. Despite the fact that such conditions are frequent, the cellular and molecular mechanisms underlying the effect of hypoxia, whether constant or intermittent, are not well understood.

Genomic signatures of human versus avian influenza A viruses.

Position-specific entropy profiles created from scanning 306 human and 95 avian influenza A viral genomes showed that 228 of 4591 amino acid residues yielded significant differences between these 2 viruses. We subsequently used 15,785 protein sequences from the National Center for Biotechnology Information (NCBI) to assess the robustness of these signatures and obtained 52 "species-associated" positions. Specific mutations on those points may enable an avian influenza virus to become a human virus.

Prognostic value of humbug gene overexpression in stage II colon cancer.

Overexpression of aspartyl (asparaginyl) beta-hydroxylase (AAH) has been demonstrated in hepatocellular carcinoma, cholangiocarcinoma, and pancreatic carcinoma. AAH has an important role in regulating cell motility and invasiveness. Humbug is a truncated homolog of AAH, with a role in calcium regulation.

Roles for the Drs2p-Cdc50p complex in protein transport and phosphatidylserine asymmetry of the yeast plasma membrane.

Drs2p, a P-type adenosine triphosphatase required for a phosphatidylserine (PS) flippase activity in the yeast trans Golgi network (TGN), was first implicated in protein trafficking by a screen for mutations synthetically lethal with arf1 (swa). Here, we show that SWA4 is allelic to CDC50, encoding a membrane protein previously shown to chaperone Drs2p from the endoplasmic reticulum to the Golgi complex. We find that cdc50Delta exhibits the same clathrin-deficient phenotypes as drs2Delta, including delayed transport of carboxypeptidase Y to the vacuole, mislocalization of resident TGN enzymes and the accumulation of aberrant membrane structures.

Exploring the mode-of-action of bioactive compounds by chemical-genetic profiling in yeast.

Discovering target and off-target effects of specific compounds is critical to drug discovery and development. We generated a compendium of "chemical-genetic interaction" profiles by testing the collection of viable yeast haploid deletion mutants for hypersensitivity to 82 compounds and natural product extracts. To cluster compounds with a similar mode-of-action and to reveal insights into the cellular pathways and proteins affected, we applied both a hierarchical clustering and a factorgram method, which allows a gene or compound to be associated with more than one group.

[Effect of insulin on expression of whole-blood platelet membrane P-selectin].

Objective: To investigate the effects of insulin on the expression of whole-blood platelet membrane P-selectin.

Methods: Flow cytometry (FCM) was used to detect the expression of whole-blood platelet membrane P-selectin, and the effect of insulin on the expression on quiescent and activated plasma platelet observed in normal young subjects.

Results: Insulin had no effect on the P-selectin expression on quiescent platelet membrane, but significantly inhibited the up-regulation of P-selectin expression induced by thrombin and collagen respectively, exhibiting dose- and time-dependent effects.

Drs2p-coupled aminophospholipid translocase activity in yeast Golgi membranes and relationship to in vivo function.

Aminophospholipid translocases (APLTs) are defined primarily by their ability to flip fluorescent or spin-labeled derivatives of phosphatidylserine (PS) and phosphatidylethanolamine (PE) from the external leaflet of a membrane bilayer to the cytosolic leaflet and are thought to establish phospholipid asymmetry in biological membranes. The identities of APLTs remain unknown, although candidate proteins include the Drs2p/ATPase II subfamily of P-type ATPases. Drs2p from budding yeast localizes to the trans-Golgi network (TGN), and here we show that this membrane contains an ATP-dependent APLT that flips 7-nitro-2-1,3-benzoxadiazol-4-yl (NBD) PS and PE derivatives from the luminal to the cytosolic leaflet.

[Effect of insulin on the expression of platelet membrane glycoprotein II b/III a and its mechanisms].

Objective: To investigate the effects of insulin on the expression of platelet membrane glycoprotein (GP) IIb/III a and its mechanism in normal subjects.

Methods: Flow cytometry was used to detect the expression of the whole-blood platelet membrane GP b/ a. The respective effects of insulin, L-nitro-arginine methyl ester (L-NAME) and methylene blue (MB) and their combination on the expression of GP b/ a were also studied.