Research progress of postoperative cognitive dysfunction in cardiac surgery under cardiopulmonary bypass.

Cardiopulmonary bypass (CPB) is often used in cardiothoracic surgery because its nonphysiological state causes pathophysiological changes in the body, causing multiorgan and multitissue damage to varying degrees. Postoperative cognitive dysfunction (POCD) is a common central nervous system complication after cardiac surgery. The etiology and mechanism of POCD are not clear.

[Protective effect of Lindera aggregata on lipopolysaccharide-induced mice acute respiratory distress syndrome by regulating p38MAPK/ERK pathway].

Objective: To explore the protective effect of Lindera aggregata on acute respiratory distress syndrome (ARDS) induced by lipopolysaccharide (LPS) in mice and its possible mechanism.

Methods: Forty C57BL/6 mice were randomly divided into sham operation group, ARDS model group, low-dose Lindera aggregata (L-LA) group and high-dose Lindera aggregata (H-LA) group, with 10 mice in each group. ARDS model was established by injecting 5 mg/kg LPS through the trachea.

Asthma attacks: Patients who survived paraquat poisoning.

A patient who survived acute paraquat (PQ) poisoning for more than 5 years was followed up in the emergency room. The patient had recurrent coughing and wheezing one month after discharge. Re-examination of chest CT showed increased dual lung texture.

Xuebijing Injection Ameliorates HS-Induced Acute Respiratory Distress Syndrome by Promoting Claudin-5 Expression.

Objective: To investigate the protective effects and underlying mechanisms of Xuebijing Injection (XBJ) on the lung endothelial barrier in hydrogen sulfide (HS)-induced acute respiratory distress syndrome (ARDS).

Methods: Sprague-Dawley rats were exposed to HS (300 ppm) to establish ARDS model, while human pulmonary microvascular endothelial cells (HPMECs) were incubated with NaHS (a HS donor, 500 µmol/L) to establish cell model. HS and XBJ were concurrently administered to the rat and cell models.

[Two case reports: chronic obstructive pulmonary disease developed in long term survived patients after acute paraquat poisoning].

Patients with acute paraquat poisoning mostly die of respiratory failure, and the surviving ones may live with pulmonary fibrosis, but the long-term changes in lung function are still un-clear. Two patients with acute paraquat poisoning survived for more than 7 years were followed up in Northern Jiangsu People's Hospital to evaluate the imaging and spirometric changes. Eight years after poisoning, 1 patient's chest computerized tomography (CT) revealed interstitial pneumonia, chronic bronchitis, emphysema, accompanied by bronchiectasis.

The role and mechanism of the annexin A1 peptide Ac2-26 in rats with cardiopulmonary bypass lung injury.

The main causes of lung injury after cardiopulmonary bypass (CPB) are systemic inflammatory response syndrome (SIRS) and pulmonary ischaemia-reperfusion injury (IR-I). SIRS and IR-I are often initiated by a systemic inflammatory response. The present study investigated whether the annexin A1 (ANX-A1) peptidomimetic Ac2-26 by binding to formyl peptide receptors (FPRs) inhibit inflammatory cytokines and reduce lung injury after CPB.

Melatonin enhances the cadmium tolerance of mushrooms through antioxidant-related metabolites and enzymes.

Many wild mushroom species are known to immune heavy metals. However, the mechanism by which this occurs remains largely unsolved. Melatonin (MT) has been proven to play an important defensive role against various abiotic stresses in plants and animals.

Involvement of claudin-5 in HS-induced acute lung injury.

Acute exposure to hydrogen sulfide (HS) can cause fatal acute lung injury (ALI). However, the mechanisms of HS-induced ALI are still not fully understood. This study aims to investigate the role of the tight junction protein claudin-5 in HS-induced ALI.

Validation of Reference Genes for Gene Expression Normalization in RAW264.7 Cells under Different Conditions.

RAW264.7 is a macrophage strain derived from mice tumour and shows a significant ability in antigen uptake. Real-time quantitative PCR (RT-qPCR) is one of the most commonly used methods in gene studies and requires suitable reference genes to normalize and quantitate the expression of gene of interest with sensitivity and specificity.

Selection and validation of appropriate reference genes for real-time quantitative PCR analysis in Momordica charantia.

Real time quantitative reverse transcription PCR (RT-qPCR) has been attracting more attention for its high sensitivity in gene expression analysis. Given the widely use of RT-qPCR in normalization, it is playing a pivotal role for seeking suitable reference genes in different species. In current work, 12 candidate reference genes including Actin 2 (ACT2), Cyclophilin 2 (CYP2), Glyceraldehyde-3-phosphate dehydrogenase C2 (GAPC2), Elongation factor 1-α (EF1-α), Nuclear cap binding protein 20 (NCBP20), Serine/threonine-protein phosphatase PP2A (PP2A), Polypyrimidine tract-binding protein 1 (PTBP1), SAND family protein (SNAD), TIP41-like protein (TIP41), Tubulin beta-6 (TUB6), Ubiquitin-conjugating enzyme 9 (UBC9) and Glyceraldehyde-3-phosphatedehydrogenase (GAPDH) were screened from the transcriptome datasets of M.

Two PBDEs exposure inducing feeding depression and disorder of digestive and antioxidative system of Daphnia magna.

2,2',4,4'-tetrabrominated diphenyl ether (BDE-47) and 2,2',4,4',5-pentabromodiphenyl ether (BDE-99) are two typical polybrominated diphenyl ethers (PBDEs), and studies have proven that these PBDs can disrupt the behaviors and physical function of aquatic organisms. However, little is known about the compositional impacts of BDE-47/BDE-99 compound pollution on the feeding behavior of Daphnia magna. In this study, a response surface methodology (RSM) was introduced into the combined toxicity assessment of BDE-47 and BDE-99 on the feeding depression of D.

A meta-analysis of early oral refeeding and quickly increased diet for patients with mild acute pancreatitis.

Background/aim: The objective of the study is to clarify whether early oral refeeding (EORF) and quickly increasing diet (QID) are of benefit to patients with mild acute pancreatitis compared with a traditional oral refeeding strategy.

Materials And Methods: Studies were searched in PubMed, Cochrane library, ScienceDirect, SpringerLink, China Biology Medicine disc and Embase. A meta-analysis was then performed, using relapse of abdominal pain, nausea/vomiting, and length of hospital stay (LOHS) as the evaluation indices.

Chemical nanosensors based on molecularly-imprinted polymers doped with silver nanoparticles for the rapid detection of caffeine in wastewater.

Caffeine is a common pharmaceutical and personal care product pollutant in wastewater. This work offers rapid and single-step detection of caffeine in an aquatic matrix based on high performance surface-enhanced Raman scattering (SERS). Novel chemical SERS nanosensors were developed employing molecularly-imprinted polymer (MIP) particles loaded with Ag nanoparticles (AgNPs) using precipitation polymerization to form AgNPs@MIP nanocomposites.

Selection and Evaluation of Appropriate Reference Genes for RT-qPCR Normalization of Gene Expression under Different Conditions.

, commonly referred to as Chinese (paddy straw) mushroom, is a basidiomycete with a protein-rich volva and pileus. Selecting appropriate reference genes is a crucial step in the normalization of quantitative real-time PCR data. Therefore, 12 candidate reference genes were selected from the transcriptome based on previous studies and then BestKeeper, geNorm, and NormFinder were used to identify reference genes stably expressed during different developmental stages and conditions.

Baicalin attenuates angiotensin II-induced endothelial dysfunction.

Angiotensin II (Ang II) has been shown to activate multiple downstream pathways resulting in endothelial dysfunction and oxidative stress. Baicalin, a natural flavone, exerts anti-oxidant and anti-apoptotic effects in cardiovascular diseases. In the present study, we hypothesized that baicalin has beneficial effects in Ang II-induced endothelial cells injury.

Hydrophobic-hydrophilic monolithic dual-phase layer for two-dimensional thin-layer chromatography coupled with surface-enhanced Raman spectroscopy detection.

Hydrophobic-hydrophilic monolithic dual-phase plates have been prepared by a two-step polymerization method for two-dimensional thin-layer chromatography of low-molecular-weight compounds, namely, several dyes. The thin 200 μm poly(glycidyl methacrylate-co-ethylene dimethacrylate) layers attached to microscope glass plates were prepared using a UV-initiated polymerization method within a simple glass mold. After cutting and cleaning the specific area of the layer, the reassembled mold was filled with a polymerization mixture of butyl methacrylate and ethylene dimethacrylate and subsequently irradiated with UV light.

[Determination of four insecticide residues in honey and royal jelly by gas chromatography-negative chemical ionization mass spectrometry].

A method was developed for the determination of four insecticide residues in honey and royal jelly by gas chromatography-negative chemical ionization mass spectrometry (GC-NCI/MS). The honey and royal jelly samples were treated with different preparation methods as the result of the different components. The honey sample was extracted with ethyl acetate and cleaned up with primary second amine, and the royal jelly sample was extracted with acetonitrile-water (1:1, v/v), and cleaned up with a C18 solid-phase extraction column.

[Value of arterial lactic acid and buffer excess in predicting the prognosis of patients with paraquat poisoning].

Objective: To investigate the early prognostic values of arterial lactate and base excess (BE) in patients with paraquat poisoning.

Methods: Seventy-five patients with paraquat poisoning were divided into sudden death group (n = 10) who died within 24 h after admission, recent death group (n = 31) who died more than 24 h after admission, and survival group (n = 34). Arterial lactate and BE were measured on admission and at 24 h after admission.

[Effect and mechanism of gastrodin in relaxing isolated thoracic aorta rings in rats].

Objective: To investigate the effect of gastrodin in relaxing isolated thoracic aorta rings in rats and discuss its possible mechanism.

Method: Isotonic tension of isolated thoracic aortic rings in rats with norepineprine (NE) and KCl was recorded to observe the vasodilatory effect of gastrodin and the influence of various drugs on it.

Result: Gastrodin had the effect in relaxing thoracic aortas with or without endothelium, and there was no significant difference.

[Vasodilation effect of EUL on rat thoracic artery].

Objective: To observe vasodilation effect of EUL and its mechanism.

Method: Isometric tension was recorded in isolated rat thoracic artery precontracted by noradrenaline (NE) to study the vasodilation effect of EUL, and to investigate the role of endothelial cell and vascular smooth muscle cell on vasodilation.

Result: EUL was shown to significantly relax the endothelium-intact arteries precontracted by NE, relaxation had fall down on endothelium-denuded arteries.